漠斑牙鲆肌肉氨基酸和脂肪酸组成分析与营养品质评价

doi:10.7506/spkx1002-6630-201315062

食品科学

漠斑牙鲆肌肉氨基酸和脂肪酸组成分析与营养品质评价

许建和1，秦洁2，邹宽1，马欢迎1,3

1.淮海工学院江苏省海洋生物技术重点实验室，江苏连云港 222000；2.上海海洋大学水产与生命学院，
上海 215104；3.淮安通威水产饲料有限公司，江苏淮安 223005

出版日期:2013-08-15 发布日期:2013-09-03
通讯作者: 许建和
基金资助:
江苏省自然科学基金项目(BK2009652)；江苏省教育厅高校自然科学基金项目(11KJD170001)；
江苏省海洋生物技术重点实验室开放课题(2007HS001)

Amino Acid and Fatty Acid Compositions and Nutritional Quality of Muscle in Southern Flounder, Paralichthys lethostigma

XU Jian-he1，QIN Jie2，ZOU Kuan1，MA Huan-ying1,3

1. Key Laboratory of Marine Biotechnology of Jiangsu Province, Huaihai Institute of Technology, Lianyungang 222000, China；
2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 215104, China；
3. Huai’an Tongwei Aquafeed Limited Company, Huai’an 223005, China

Online:2013-08-15 Published:2013-09-03
Contact: XU Jian-he

摘要/Abstract

摘要：

对人工养殖漠斑牙鲆肌肉进行一般营养组分、氨基酸和脂肪酸组成分析，并对其营养品质进行评价。结果表明：漠斑牙鲆肌肉鲜样中粗蛋白和粗脂肪含量分别为17.74%和3.02%。肌肉粗蛋白中检测出19种氨基酸，其中谷氨酸(120.4mg/g pro)、天冬氨酸(75.4mg/g pro)、甘氨酸(73.6mg/g pro)、亮氨酸(72.3mg/g pro)、赖氨酸(67.8mg/g pro)和精氨酸(63.4mg/g pro)含量较高。依据世界粮农组织/世界卫生组织/联合国大学(FAO/WHO/UNU)必需氨基酸基准模式，10种必需氨基酸评分均大于100，表明漠斑牙鲆肌肉蛋白是氨基酸平衡性较好的优质蛋白。脂肪酸检测结果显示漠斑牙鲆肌肉中饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸含量分别为32.23%、30.79%和27.01%。n-3系列多不饱和脂肪酸中二十碳五烯酸(EPA，C20:5n-3)和二十二碳六烯酸(DHA，C22:6n-3)含量较高，为7.38%和12.07%；n-6系列多不饱和脂肪酸中花生四稀酸(AA，C20:4n-6)含量最高，为4.49%。n-6和n-3系列多不饱和脂肪酸比值为0.35，远低于我国和英国卫生部推荐的4.0最高安全限值。

关键词: 漠斑牙鲆, 一般营养组分, 脂肪酸, 氨基酸, 营养评价

Abstract:

The proximate composition and amino acid and fatty acid profiles of muscle in cultured southern flounder,
Paralichthys lethostigma, were analyzed to evaluate its nutritional quality. Southern flounder muscle contained 17.74% crude
protein and 3.02% crude lipid. In addition, Nineteen amino acids were detected, including a large amount of glutamic acid
(120.4 nmg/g crude protein), aspartic acid (75.4 mg/g crude protein), glycine (73.6 mg/g crude protein), leucine (72.3 mg/g
crude protein), lysine (67.8 mg/g crude protein) and arginine (63.4 mg/g crude protein). According to the reference amino acid
pattern recommended by FAO/WHO/UNU (1985) for pre-school children (2 to 5 years old), the scores for all the essential
amino acids were over 100 indicating the muscle of southern flounder to contain high-quality protein with a good amino acid
balance. Total saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid (PUFA) contents in the muscle of
southern flounder were 32.23%, 30.79% and 27.01%, respectively. In n-3 PUFAs, 7.38% eicosapentaenoic acid (EPA, C20:5n-3)
and 12.07% docosahexaenoic acid (DHA, C22:6n-3) were detected, while 4.49% arachidonic acid (AA, C20:4n-6) was detected in n-6
PUFAs. The ratio of ΣPUFA n-6/ΣPUFA n-3 was 0.35, which was significantly lower than the maximum safety value of 4.0
recommended by the UK Department of Health.

Key words: southern flounder Paralichthys lethostigma, proximate composition, fatty acid, amino acid, nutritional assessment

中图分类号:

TS254.1

许建和1，秦洁2，邹宽1，马欢迎1,3. 漠斑牙鲆肌肉氨基酸和脂肪酸组成分析与营养品质评价[J]. 食品科学, doi: 10.7506/spkx1002-6630-201315062.

XU Jian-he1，QIN Jie2，ZOU Kuan1，MA Huan-ying1,3. Amino Acid and Fatty Acid Compositions and Nutritional Quality of Muscle in Southern Flounder, Paralichthys lethostigma[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201315062.

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漠斑牙鲆肌肉氨基酸和脂肪酸组成分析与营养品质评价

Amino Acid and Fatty Acid Compositions and Nutritional Quality of Muscle in Southern Flounder, Paralichthys lethostigma

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