荧光分光光度法对黄酒氧自由基吸收能力检测方法的建立

doi:10.7506/spkx1002-6630-201318038

摘要/Abstract

摘要：

目的：建立用荧光分光光度计测定黄酒氧自由基吸收能力(ORAC)的改进方法。方法：以市售黄酒为样品，使用荧光分光光度计，以荧光半数衰减时间为观察指标，通过Trolox标准曲线，换算得到黄酒的ORAC值，并测定最低检测限、重复性精密度、平行性精密度、加标回收率等。结果：激发波长为490nm，发射波长为517nm。相对荧光半数衰减时间与Trolox浓度的线性回归方程为Y=7.2357X－0.8189，线性决定系数R2为0.9948，检测范围0.28～8.00μmol/L，方法的重复性精密度为3.02%，平行性精密度为2.69%，加标回收率在93.00%～102.00%之间。检测样品的ORAC值为4.68～5.21μmol/mL。结论：ORAC荧光分光光度法简化了操作步骤，缩短了实验时间，同时保证了良好的准确度、精密度和稳定性，适用于测定不同品牌不同年份黄酒的ORAC。

关键词: 黄酒, 发酵酒, 氧自由基吸收能力, 荧光素, 荧光分光光度法

Abstract:

Objective: To establish a modified method for the determination of oxygen radical absorbance capacity (ORAC)
in Chinese rice wine by fluorescence spectrophotometry. Methods: Commercial samples of Chinese rice wine were
determined by a fluorescence spectrophotometer for fluorescence half-decay time. As a result, a Trolox calibration curve
was developed and used to calculate ORAC. The analytical method was validated with respect to limit of detection (LOD),
precision for repeated and parallel determinations and spiked recovery. Results: The excitation wavelength was 490 nm,
and emission wavelength was 517 nm. The regression equation between relative fluorescence half-decay time and Trolox
concentration was Y = 7.2357X － 0.8189 with R2 of 0.9948 and detection range of 0.28–8.00 μmol Trolox/L. The precision
for 5 repeated and 5 parallel determinations were 3.02% and 2.69%, respectively. The recovery rates of spiked samples
were in the range of 93.00%–102.00%. The ORAC value of Chinese rice wine was 4.68–5.21 μmol Trolox/mL. Conclusion:
Fluorescence spectrophotometry is a simple, time-saving, accurate, precise and stable method which is useful to determine
ORAC in Chinese rice wines of different ages and from different brands

Key words: Chinese rice wine, fermented alcoholic beverage, oxygen radical absorbance capacity (ORAC), fluorescein(FL), fluorescence spectrophotometry

黄玥，陆文蔚，唐立伟，黄灵洁，白晨. 荧光分光光度法对黄酒氧自由基吸收能力检测方法的建立[J]. 食品科学, doi: 10.7506/spkx1002-6630-201318038.

HUANG Yue，LU Wen-wei，TANG Li-wei，HUANG Ling-jie，BAI Chen. Determination of Oxygen Radical Absorbance Capacity in Chinese Rice Wine by Fluorescence Spectrophotometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201318038.

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荧光分光光度法对黄酒氧自由基吸收能力检测方法的建立

Determination of Oxygen Radical Absorbance Capacity in Chinese Rice Wine by Fluorescence Spectrophotometry

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