食品科学 ›› 2013, Vol. 34 ›› Issue (23): 291-295.doi: 10.7506/spkx1002-6630-201323058

• 营养卫生 • 上一篇    下一篇

青钱柳多糖对3T3-L1前脂肪细胞增殖分化的影响

刘 姚,傅凌韵,叶振南,李 楠,王文君*   

  1. 江西农业大学 南昌市农产品加工与质量控制重点实验室,江西 南昌 330045
  • 收稿日期:2013-06-26 修回日期:2013-11-04 出版日期:2013-12-15 发布日期:2014-01-03
  • 通讯作者: 王文君 E-mail:wwjun9999@sina.com
  • 基金资助:

    国家自然科学基金项目(31160319);江西省科技厅工业攻关项目(2009BGA01900);
    江西省教育厅科技计划项目(KJLD13027;GJJ13281)

Effect of Cyclocarya paliurus (Batal.) Iljinskaja Polysaccharide on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

LIU Yao,FU Ling-yun,YE Zhen-nan,LI Nan,WANG Wen-jun*   

  1. Key Laboratory for Agricultural Product Processing and Quality Control of Nanchang City, Jiangxi Agricultural University,
    Nanchang 330045, China
  • Received:2013-06-26 Revised:2013-11-04 Online:2013-12-15 Published:2014-01-03
  • Contact: WANG Wen-jun E-mail:wwjun9999@sina.com

摘要:

为探讨青钱柳多糖对3T3-L1前脂肪细胞增殖分化的影响。以青钱柳多糖为研究对象,通过D301-R大孔吸附树脂和DEAE纤维素阴离子树脂分离纯化,得到青钱柳多糖组分(CPC),采用凝胶渗透色谱(GPC)和多角度极光散射(MALLS)法分析其分子质量;采用不同质量浓度的多糖作用于3T3-L1脂肪细胞,采用MTT法和油红O染色检测多糖对脂肪细胞增殖分化的影响,同时测定细胞葡萄糖的消耗和细胞外甘油含量。结果表明:CPC含有3个组分,其黏均分子质量(Mη)分别为50300、3951g/mol和1406g/mol;在一定的质量浓度范围内,CPC对前脂肪细胞的生长表现为增殖趋势,且呈现一定的量效关系;与对照组相比,青钱柳多糖能够降低3T3-L1脂肪细胞对葡萄糖的消耗,降低3T3-L1脂肪细胞细胞外甘油含量,其抑制率分别高达9.61% 和30.8%。提示青钱柳多糖在一定质量浓度范围能够促进脂肪细胞的增殖,降低葡萄糖的消耗和细胞外甘油含量。

关键词: 青钱柳多糖;3T3-L1脂肪细胞;黏均分子质量(M&eta, );增殖;分化

Abstract:

This study was conducted to investigate the effect of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide
on the proliferation and differentiation of 3T3-L1 preadipocytes. The crude polysaccharide extract of C. paliurus was
fractionated by using D301-R macroporous resin and DEAE-anion fiber resin, and a C. paliurus polysaccharide component
(CPC) was obtained. Gel permeation chromatography (GPC) and multi-angle laser light scattering (MALLS) were then used
to determine its molecular weight distribution. The proliferation and differentiation of 3T3-L1 preadipocytes treated with
different CPC concentrations were measured by MTT and oil red O staining, respectively. At the same time the glucose
consumption and extracellular glycerol content were determined. Results showed that CPC consisted of three different
components and their viscosity-average molecular weight (Mη) was determined to be 50300, 3951 g/mol and 1406 g/mol,
respectively. Within a certain concentration range, CPC affected the proliferation of preadipocytes in a concentrationdependent
manner. Compared with the control group, CPC could reduce the glucose consumption and extracellular glycerin
content of 3T3-L1 adipocytes with inhibition ratios of as high as 9.61% and 30.8% respectively. These observations reveal
that the C. paliurus polysaccharide is capable of promoting the proliferation of adipocytes in certain concentration range and
reducing glucose consumption and extracellular glycerin content.

Key words: Cyclocarya paliurus polysaccharide, 3T3-L1 adipocytes, viscosity average molecular weight, proliferation, differentiation

中图分类号: