关键词: 月见草油, 酶法, 压榨, 出油效率, 指标测定

Abstract:

In this work, evening primrose oil was pressed from enzymatically pretreated samples of evening primrose
seeds. The enzymatic pretreatment was carried out using a mixture composed of a commercial complex enzyme preparation
hydrolyzing carbohydrates, viscozyme and cellulase (all from Novozymes, 4:1:1). By using one-factor-at-a-time and
orthogonal array design methods, the optimal enzymatic pretreatment conditions were determined to be hydrolysis for 5.5 h at 50 ℃,
initial pH 5.0 with a total enzyme dosage of 2%. Under these conditions, the yield of free oil was 24%. The pretreated material
was dried until the moisture content reached 9%, and then pressed initially at 60 ℃, finally at 90 ℃ and 3.5 MPa, resulting in an oil
yield of 69%. γ-Linolenic, linoleic and some other unsaturated fatty acids were retained at significant levels in the evening
primrose oil, and the oil quality was good.

Key words: evening primrose oil, enzymatic method, pressing, oil yield, determination of quality parameters