食品科学 ›› 2014, Vol. 35 ›› Issue (2): 96-101.doi: 10.7506/spkx1002-6630-201402018

• 工艺技术 • 上一篇    下一篇

酶法预处理压榨月见草油工艺的研究

王俊国1,张 欢2,刘 飞2,刘丹怡2,潘明喆2,*,于殿宇2   

  1. 1.吉林工商学院 粮油食品深加工吉林省高校重点实验室,吉林 长春 130062;
    2.东北农业大学食品学院,黑龙江 哈尔滨 150030
  • 收稿日期:2013-09-01 修回日期:2013-12-23 出版日期:2014-01-25 发布日期:2014-02-19
  • 通讯作者: 潘明喆 E-mail:dyyu2000@126.com
  • 基金资助:

    粮油食品深加工吉林省高校重点实验室基金项目(2013006);黑龙江省科技计划项目(GC13B207)

Optimization of Enzymatic Pretreatment Process for Pressing of Evening Primrose Oil

WANG Jun-guo1, ZHANG Huan2, LIU Fei2, LIU Dan-yi2, PAN Ming-zhe2,*, YU Dian-yu2   

  1. 1. Key Laboratory of Grain and Oil Processing of Jilin Province, Jilin Business and Technology College,
    Changchun 130062, China; 2. School of Food, Northeast Agricultural University, Harbin 150030, China
  • Received:2013-09-01 Revised:2013-12-23 Online:2014-01-25 Published:2014-02-19
  • Contact: PAN Ming-zhe E-mail:dyyu2000@126.com

摘要:

以月见草籽为原料,采用酶法预处理后再压榨的方法制取月见草油,先用复合酶(复合多糖酶、植物水 解酶、纤维素酶比为4∶1∶1)对月见草籽进行前处理,通过单因素和正交试验确定最佳酶解条件为酶解温度50 ℃、 酶解pH 5.0、酶用量2%、酶解时间5.5 h,在此条件下游离油得率24%,然后将含油物料烘干后调整水分至9%,以 60 ℃为初始入榨温度进行压榨,最后得出在压榨温度90 ℃、压榨压力3.5 MPa时,出油率可达69%。制取得到的月 见草油中的γ-亚麻酸以及亚油酸等不饱和酸被很好的保留,油脂品质良好。

关键词: 月见草油, 酶法, 压榨, 出油效率, 指标测定

Abstract:

In this work, evening primrose oil was pressed from enzymatically pretreated samples of evening primrose
seeds. The enzymatic pretreatment was carried out using a mixture composed of a commercial complex enzyme preparation
hydrolyzing carbohydrates, viscozyme and cellulase (all from Novozymes, 4:1:1). By using one-factor-at-a-time and
orthogonal array design methods, the optimal enzymatic pretreatment conditions were determined to be hydrolysis for 5.5 h at 50 ℃,
initial pH 5.0 with a total enzyme dosage of 2%. Under these conditions, the yield of free oil was 24%. The pretreated material
was dried until the moisture content reached 9%, and then pressed initially at 60 ℃, finally at 90 ℃ and 3.5 MPa, resulting in an oil
yield of 69%. γ-Linolenic, linoleic and some other unsaturated fatty acids were retained at significant levels in the evening
primrose oil, and the oil quality was good.

Key words: evening primrose oil, enzymatic method, pressing, oil yield, determination of quality parameters

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