贮藏温度对哈密瓜蔗糖代谢途径中14种酶活性的影响

doi:10.7506/spkx1002-6630-201402053

食品科学 ›› 2014, Vol. 35 ›› Issue (2): 271-276.doi: 10.7506/spkx1002-6630-201402053

贮藏温度对哈密瓜蔗糖代谢途径中14种酶活性的影响

蔡修臻，庞广昌*

天津商业大学生物技术与食品科学学院，天津市食品生物技术重点实验室，天津 300134

收稿日期:2013-06-27 修回日期:2013-12-09 出版日期:2014-01-25 发布日期:2014-02-19
通讯作者: 庞广昌 E-mail:952597220@qq.com
基金资助:
国家自然科学基金面上项目（30871951）；天津市科技支撑计划项目（10ZCKFNC01800）

Effects of Different Storage Temperatures on the Activities of Fourteen Enzymes Involved in Sucrose Metabolism in Hami Melon

CAI Xiu-zhen, PANG Guang-chang*

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
Tianjin University of Commerce, Tianjin 300134, China

Received:2013-06-27 Revised:2013-12-09 Online:2014-01-25 Published:2014-02-19
Contact: PANG Guang-chang E-mail:952597220@qq.com

摘要/Abstract

摘要：

为了系统研究哈密瓜在不同贮藏温度条件下的蔗糖积累和呼吸代谢情况，在不同贮藏温度（0、5、10、 15、20、25、30、35、40、45 ℃）测定哈密瓜外果皮、内果皮、外果肉、中果肉、内果肉5 个部位蔗糖、乳酸、呼吸代谢途径中14 种酶的基因表达活性及其变化情况。结果表明：哈密瓜果实5 个部位中蔗糖转化酶和乳酸脱氢酶活性最高，其活性大小依次为：内果皮＞外果肉＞外果皮＞中果肉＞内果肉；哈密瓜在5 ℃贮藏时，包括蔗糖转化酶和乳酸脱氢酶的14 种酶的活性均处于最低水平，可见5 ℃可有效地控制蔗糖代谢，是哈密瓜的最适贮藏温度。

关键词: 贮藏温度, 哈密瓜, 蔗糖代谢, 酶活性, 呼吸作用

Abstract:

This study aimed to shed light on the respiratory metabolism and sucrose accumulation in Hami melon under
different storage conditions. The activities (gene expression levels) of fourteen enzymes involved in sucrose metabolism
were measured at different storage temperatures (0, 5, 10, 15, 20, 25, 30, 35, 40 and 45 ℃). These enzymes in the epicarp,
endocarp, outer pulp, middle pulp, inner pulp were investigated as well. The results showed that among these enzymes
examined, the activities of invertase and lactate dehydrogenase were the highest. The decreasing order of the two enzyme
activities in five tissues was as follows: endocarp ＞ outer pulp ＞ epicarp ＞ middle pulp ＞ inner pulp. When Hami melon
was stored at 5 ℃, the activities of all 14 enzymes were at a low level. Thus, 5 ℃ is the optimal storage temperature and
sugar metabolism can be controlled effectively at this temperature.

Key words: storage temperature, Hami melon, sucrose metabolism, enzyme activity, respiration

中图分类号:

TS255.1

蔡修臻，庞广昌*. 贮藏温度对哈密瓜蔗糖代谢途径中14种酶活性的影响[J]. 食品科学, 2014, 35(2): 271-276.

CAI Xiu-zhen, PANG Guang-chang*. Effects of Different Storage Temperatures on the Activities of Fourteen Enzymes Involved in Sucrose Metabolism in Hami Melon[J]. FOOD SCIENCE, 2014, 35(2): 271-276.

参考文献

[1] 张保才.“风味4号”甜瓜果实糖积累及采后生理代谢研究[D]. 武汉：华中农业大学，2010.

[2] 斯越秀,胡妙君,汪财生等．贮藏温度对哈密瓜“黄皮9818”采后营养品质的影响［J］.江苏农业科学,2012,40（2):210-212．

[3] Ji luan Chen, Jing Zhang, Lijun Song.Changes in microorganism, enzyme, aroma of hami melon (Cucumis melo L.) juice

treated with dense phase carbon dioxide and stored at 4℃[J]. Innovative Food Science and Emerging Technologies , 2010,11: 623– 629.

[4] 李萍,车凤斌,胡柏文.气调贮藏不同气体比例对哈密瓜86-1贮期品质及生理活性的影响[J].新疆农业科学,2010,47(1):104 -109.

[5] 胡少华,张立宇,杨超等.哈密瓜涂膜保鲜技术应用初报[J].农村科技,2010,4:55-56.

[6] Fengsong Cong, Yungui Zhang , Wenyan Dong.Use of surface coatings with natamycin to improve the storability

of Hami melon at ambient temperature[J]. Postharvest Biology and Technology, 2007,46: 71–75.

[7] 吴明辉.新疆哈密瓜保鲜技术的研究与应用[D]. 乌鲁木齐：新疆大学,2008.

[8] 王静,王晓鹏,妥丽敏.哈密瓜采前套袋对贮期腐烂的影响[J].果树学报2013,30(3):465～469.

[9] Annick Moing, Asaph Aharoni, Benoit Biais. Extensive metabolic cross-talk in melon fruit revealed by spatial and developmental combinatorial metabolomics[J]. New Phytologist, 2011,1-14.

[10] Pierce V A, Crawford D L. Phylogenetic Analysis of Thermal Acclimation of the Glycolytic Enzymes in the Genus Fundulus[J].Physiol Zool,1997,70(6): 597-609.

[11] Valerie A, Pierce. Rapid enzyme assays investigating the variation in the glycolytic pathway in field-caught populations of Fundulus heteroclitus [J].Biochemical Genetic.1994,32:317-330.

[12] Lester GE, Arias LS, Lim MG. Muskmelon fruit soluble acid invertase and sucrose Phosphate synthase activity and polypeptide profiles during growth and maturation. J Amer Soc Hortic Sci,2001,126:33一36.

[13] 王贤磊,高兴旺,李冠.甜瓜遗传图谱的构建及果实与种子QTL分析[J].遗传,2011,33(12): 1398―1408.

[14] 张明方,李志凌,陈坤松.网纹甜瓜发育果实糖分积累与蔗糖代谢参与酶的关系[J].植物生理与分子生物学学报,2003,29: 455一462.

[15] Bi Yang, Tian Shiping, Lui Hongxia, et al.Effect of temperature on chilling injury,decay and quality of Hami melon during storage[J].Postharvest Biology and Technology,2003,29( 2) :341－348.

[16] Matsuura Endo C,Kobayashi A,Noda T,et al. Changes in sugar content and activity of vacuolar acid invertase during low temperature storage of potato tubers from six Japanese cultivars[J]. Journal of Plant Research,2004, 117: 131 - 137.

[17] 王幼宁,刘孟雨,李霞.植物3-磷酸甘油醛脱氢酶的多维本质[J].西北植物学报,2005,25;607—614.

[18] Roberto Coppari. Metabolic actions of hypothalamic SIRT1[J]. Trends in Endocrinology and Metabolism,2012, 23(4):179-185.

[19] Subir K. Roy Chowdhury, Rick T. Dobrowsky, Paul Fernyhough, Nutrient excess and altered mitochondrial proteome and function contribute to neurodegeneration in diabetes[J]. Mitochondrion, 2011, 11:845–854.

贮藏温度对哈密瓜蔗糖代谢途径中14种酶活性的影响

Effects of Different Storage Temperatures on the Activities of Fourteen Enzymes Involved in Sucrose Metabolism in Hami Melon

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