野生蓝果忍冬多酚鉴定及其抗氧化、降血糖活性

doi:10.7506/spkx1002-6630-20200709-123

食品科学 ›› 2021, Vol. 42 ›› Issue (11): 47-55.doi: 10.7506/spkx1002-6630-20200709-123

野生蓝果忍冬多酚鉴定及其抗氧化、降血糖活性

乔锦莉，张妍，刘佩，郭良川，霍俊伟

（1.东北农业大学园艺园林学院，黑龙江哈尔滨 150030；2.寒地小浆果开发利用国家地方联合工程研究中心，黑龙江哈尔滨 150030；3.东北农业大学农业农村部东北地区园艺作物生物学与种质创制重点实验室，黑龙江哈尔滨 150030）

出版日期:2021-06-15 发布日期:2021-06-29
基金资助:
“十三五”国家重点研发计划重点专项（2016YFC500304-05）；国家自然科学基金面上项目（31272130）；国家自然科学基金青年科学基金项目（31801553）；黑龙江省自然科学基金优秀青年项目（YQ2020C011）

Polyphenol Composition and Antioxidant and Hypoglycemic Activities in Wild Blue Honeysuckle Fruit

QIAO Jinli, ZHANG Yan, LIU Pei, GUO Liangchuan, HUO Junwei

(1. College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; 2. National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Harbin 150030, China; 3. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, China)

Online:2021-06-15 Published:2021-06-29

摘要/Abstract

摘要： 目的：对野生蓝果忍冬进行多酚成分鉴定和抗氧化、抗淀粉酶活性研究。方法：以我国小兴安岭地区的野生蓝果忍冬果实为材料，通过固相萃取技术分离纯化得到花色苷和非花色苷分离液，测定分离液中的化学成分，并测定多酚粗提液中总酚和总花色苷含量，抗氧化活性（1,1-二苯基-2-三硝基苯肼自由基清除能力、铁离子还原能力、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力、氧自由基吸收能力），抗α-淀粉酶、α-葡萄糖苷酶活性和脂肪酶活性。结果：野生蓝果忍冬果实中含有丰富的花色苷和非花色苷多酚，尤其是矢车菊素-3-葡萄糖苷和山柰酚-3-芸香糖苷含量很高；蓝果忍冬多酚粗提液中总酚含量为82.7 mg/100 g，总花色苷含量为49.8 mg/100 g，具有较强的抗氧化活性；对α-淀粉酶、α-葡萄糖苷酶活性的半抑制质量浓度分别为0.39 mg/mL和0.933 mg/mL，对脂肪酶活性的半抑制质量浓度为12.31 mg/mL，说明蓝果忍冬可以作为一种有前景的淀粉酶抑制剂。

关键词: 野生蓝果忍冬；多酚；抗氧化活性；抗淀粉酶活性

Abstract: The polyphenol composition and antioxidant and anti-starch hydrolase activities in wild blue honeysuckle fruit collected from the Lesser Khingan Mountains in northeast China were analyzed. Anthocyanin and non-anthocyanin fractions were obtained by solid-phase extraction (SPE) and the chemical composition was determined. Total phenolics and total anthocyanins were also determined in the polyphenol crude extracts, and antioxidant activity was measured by determining 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging capacity, ferric reducing antioxidant power (FRAP), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging capacity, and oxygen radical absorbance capacity (ORAC). In addition, the inhibitory activity of α-amylase, α-glucosidase and lipase were determined. The results showed that the fruit contained abundant anthocyanins and non-anthocyanin polyphenols, especially cyanidin-3-glucoside and kaempferol-3-rutinoside. The contents of total phenolics and total anthocyanins in the polyphenol crude extracts were up to 82.7 and 49.8 mg/100 g respectively, indicating strong antioxidant activity. The half-maximum inhibitory concentrations (IC50) for α-amylase, α-glucosidase and lipase were 0.39, 0.933 and 12.31 mg/mL, respectively. This study demonstrates that blue honeysuckle fruit could be used as a promising inhibitor of starch hydrolase.

Key words: wild blue honeysuckle; polyphenols; antioxidant activity; anti-starch hydrolase activity

中图分类号:

S663.9

乔锦莉，张妍，刘佩，郭良川，霍俊伟. 野生蓝果忍冬多酚鉴定及其抗氧化、降血糖活性[J]. 食品科学, 2021, 42(11): 47-55.

QIAO Jinli, ZHANG Yan, LIU Pei, GUO Liangchuan, HUO Junwei. Polyphenol Composition and Antioxidant and Hypoglycemic Activities in Wild Blue Honeysuckle Fruit[J]. FOOD SCIENCE, 2021, 42(11): 47-55.

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野生蓝果忍冬多酚鉴定及其抗氧化、降血糖活性

Polyphenol Composition and Antioxidant and Hypoglycemic Activities in Wild Blue Honeysuckle Fruit

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