食品科学

• 基础研究 • 上一篇    下一篇

大孔吸附树脂对草鱼肽的脱盐作用

林利美,胡勤玲,王 申,吴永宁,宫智勇   

  1. 1.武汉工业学院食品科学与工程学院,湖北 武汉 430023;2.国家食品安全风险评估中心,北京 100021
  • 出版日期:2014-03-15 发布日期:2014-04-04

Desalination of Grass Carp Peptides with Macroporous Adsorption Resin

LIN Li-mei, HU Qin-ling, WANG Shen, WU Yong-ning, GONG Zhi-yong   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
    2. China National Center for Food Safety Risk Assessment, Beijing 100021, China
  • Online:2014-03-15 Published:2014-04-04

摘要:

目的:研究DA201-C大孔吸附树脂对草鱼肽的脱盐作用。方法:对脱盐前后草鱼肽的灰分、肽含量、分子质量及氨基酸组成进行分析,采用脱盐率、血管紧张素转化酶(angiotensin I-converting enzyme,ACE)抑制活性、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)自由基清除率和铁还原抗氧化能力(ferric reducing antioxidant potential,FRAP)为指标考察其脱盐效果。结果:草鱼肽的脱盐率为82.02%,脱盐处理后,肽片段和疏水氨基酸得到有效富集;ACE抑制活性显著增大,半抑制质量浓度(IC50)由0.36 mg/mL变为0.23 mg/mL;抗氧化性增强,其IC50由5.59 mg/mL变为4.48 mg/mL;总抗氧化值由1.018增大到1.261。结论:采用DA201-C大孔吸附树脂对草鱼肽脱盐是一种简便有效的处理方法。

关键词: 大孔吸附树脂, 草鱼肽, 脱盐

Abstract:

To investigate the desalination of grass carp peptides (GCP) with macroporous adsorption resinDA201-C. Methods: Ash and peptide contents, molecular weight and amino acid composition of GCP were analyzed beforeand after desalination. Desalination efficiency, angiotensin I-converting enzyme (ACE) inhibitory activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant potential (FRAP) were used to evaluatehow well GCP was desalinated. The results showed that the desalination efficiency of GCP was 82.02%. The peptidefragments and hydrophobic amino acids were enriched in desalinated GCP. Meanwhile, the ACE inhibitory activity wassignificantly higher than that of native GCP, showing a decrease in IC50 from 0.36 to 0.23 mg/mL, and the antioxidantactivity was also increased showing an IC50 of 4.48 mg/mL compared to 5.59 mg/mL for native GCP. The desalinationresulted in an increase in the FRAP value of GCP from 1.018 to 1.261. Conclusion: The use of macroporous adsorption resinDA201-C provides a simple and effective method to desalinate GPC.

Key words: macroporous adsorption resin, grass carp peptides, desalination