食品科学

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顶空固相微萃取-气相色谱-质谱法分析东北油豆角挥发性成分

王 艳,宋述尧*,张 越,赵 靖,赵春波,张雪梅   

  1. 吉林农业大学园艺学院,吉林 长春 130118
  • 出版日期:2014-06-25 发布日期:2014-07-03
  • 通讯作者: 宋述尧 E-mail:ssyjlau@126.com
  • 基金资助:

    吉林省现代农业产业技术体系建设专项(2013026);吉林省教育厅“十二五”科学技术研究项目(2013075)

Analyses of Volatile Components of Youdoujiao (Phaseolus vulgaris L. var. chinensis Hort.) from Northeast China by HS-SPME-GC-MS

WANG Yan, SONG Shu-yao*, ZHANG Yue, ZHAO Jing, ZHAO Chun-bo, ZHANG Xue-mei   

  1. College of Horticulture, Jilin Agricultural University, Changchun 130118, China
  • Online:2014-06-25 Published:2014-07-03
  • Contact: SONG Shu-yao E-mail:ssyjlau@126.com

摘要:

采用顶空固相微萃取气相色谱-质谱法对东北油豆角挥发性物质进行检测分析,为优质栽培、品质育种及贮藏加工提供参考。结果表明,油豆角挥发成分包括醛、醇、酮、酸、酯、烃、呋喃及醚等类物质。未加工青荚中鉴定出48 种挥发成分,主要为2-己烯醛(相对含量为40.01%)、己醇(12.64%)、己醛(11.40%),与3-己烯-1-醇、反-2-己烯醛、反-2-顺-6-壬二烯醛、3-甲基-1-丁醇、2-乙基己醇、反,反-2,4-庚二烯醛、反-2-壬烯醛、β-环柠檬醛、肉豆蔻醛等物质共同作用形成青豆荚气味;可食熟荚中鉴定出26 种挥发成分,主要为1-辛烯-3-醇(48.77%),与顺-4-庚烯醛、3-甲硫基丙醛,反-2-庚烯醛、辛醛、壬醛、香叶基丙酮等共同作用形成油豆角特异
芳香性质。

关键词: 油豆角, 顶空固相微萃取, 气相色谱-质谱法, 挥发性物质

Abstract:

Youdoujiao (Phaseolus vulgaris L. var. chinensis Hort) is an excellent cultivar group of snap bean which grows
in the special natural geographical environment in northeast China. In this study, headspace solid phase microextraction
(HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) technique was applied to analyze the
volatile compounds of unprocessed and cooked Youdoujiao pods. The results indicated that the main volatile compounds
of Youdoujiao were aldehydes, alcohols, ketones, acids, esters, hydrocarbons, furans and ethers. Forty-eight volatile
compounds were identified in the unprocessed pods, mainly including 2-hexenal (40.01%) 1-hexanol (12.64%) and hexanal
(11.40%). These three compounds contributed to the odor of green pods together with 3-hexen-1-ol, (E)-2-hexenal, (E,Z)-
2,6-nonadienal, 3-methyl-1-butanol, 2-ethyl-1-hexanol, (E,E)-2,4-hexadienal, (E)-2-nonenal, 2,6,6-trimethyl-1-cyclohexene-
1-carboxaldehyde, and tetradecanal. Twenty-six volatile compounds were identified in the cooked pods. The major
compound was 1-octen-3-ol, accounting for 48.77% of the total volatile components, which contributed to the aroma of
cooked Youdoujiao pods in combination with (Z)-4-heptenal, 3-(methylthio)-propanal, (E)-2-heptenal, (E)-6,10-dimethyl-
5,9-U-ecadien-2-one, octanal, and nonanal.

Key words: Youdoujiao (Phaseolus vulgaris L. var. chinensis Hort.), headspace solid phase microextraction (HS-SPME);gas chromatography-mass spectrometry (GC-MS), volatile compounds

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