食品科学

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NO熏蒸处理对冷藏枇杷果实木质化劣变的影响

张美姿,吴光斌,陈发河*   

  1. 集美大学生物工程学院,福建 厦门 361021
  • 出版日期:2014-08-25 发布日期:2014-08-25
  • 通讯作者: 陈发河
  • 基金资助:

    国家自然科学基金面上项目(31171777);福建省科技计划重点项目(2007N0046);
    福建省自然科学基金项目(B0510026);厦门市科技计划项目(350Z20103024)

Effect of Nitric Oxide Fumigation on Lignification of Loquat Fruits during Cold Storage

ZHANG Mei-zi, WU Guang-bin, CHEN Fa-he*   

  1. College of Bioengineering, Jimei University, Xiamen 361021, China
  • Online:2014-08-25 Published:2014-08-25
  • Contact: CHEN Fa-he

摘要:

为了探讨外源一氧化氮(NO)熏蒸处理对冷藏枇杷果实木质素代谢的影响,以‘解放钟’枇杷果实为试材,采用不同水平NO(0、5、15、25、35、45 μL/L)熏蒸处理,于5 ℃条件下贮藏,测定冷藏过程中枇杷果实的硬度、出汁率、可溶性总糖、可滴定酸、木质素含量及木质素代谢相关酶活性的变化。结果表明:NO熏蒸处理可延缓可溶性总糖及可滴定酸含量的下降,并抑制硬度的上升及出汁率的降低,较好地保持果实的商品品质。NO熏蒸处理显著抑制了苯丙氨酸解氨酶、肉桂醇脱氢酶活性的上升,延缓了可溶性总糖含量的降低,且抑制了木质素含量的上升,从而延缓了冷藏枇杷果实木质化劣变进程,以15 μL/L和25 μL/L NO处理效果较为明显。

关键词: 枇杷, 木质化, 一氧化氮, 苯丙氨酸解氨酶, 肉桂醇脱氢酶

Abstract:

The present study was designed to explore the effect of exogenous nitric oxide (NO) on lignification of loquat
(Eriobotrya japonica Lindl. cv. Jiefangzhong) fruits during cold storage. The fruits were fumigated with (0, 5, 15, 25, 35
and 45 μL/L) NO gas. Fruit firmness, juice percentage, total soluble sugar, titratable acid and lignin content, and the enzyme
activities related to lignin metabolism were analyzed during cold storage (5 ℃) of loquat fruits. The results indicated that
nitric oxide treatments delayed the decline of total soluble sugar and titratable acid content. Meanwhile, NO fumigation
inhibited the increase of fruit firmness and the decrease of juice percentage, thereby maintaining better commercial quality
of loquat fruits. Nitric oxide treatments significantly inhibited the activities of phenylalanine ammonialyase (PAL) and
cinnamylalcohol dehydrogenase (CAD), delayed the reduction of total soluble sugar, and inhibited the increase of lignin
content, thus postponing the development of lignification of loquat fruits during cold storage. The treatments with nitric
oxide at 15 and 25 μL/L were more effective.

Key words: loquat fruits, lignification, nitric oxide, phenylalanine ammonialyase, cinnamylalcohol dehydrogenase

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