产黄樟素内生真菌交链孢霉LJX27的发酵条件优化

doi:10.7506/spkx1002-6630-201417032

食品科学

产黄樟素内生真菌交链孢霉LJX27的发酵条件优化

徐洲，尹礼国，张超，魏琴

1.宜宾学院生命科学与食品工程学院，四川宜宾 644000；2.发酵资源与应用四川省高校重点实验室，四川宜宾 644000

出版日期:2014-09-15 发布日期:2014-09-12

Optimization of Fermentation Conditions of Alternaria LJX27 for Safrole Production

XU Zhou, YIN Li-guo, ZHANG Chao, WEI Qin

1. College of Life Science and Food Engineering, Yi Bin University, Yibin 644000, China; 2. Key Laboratory of Fermentation
Resource and Application of Institutes of Higher Learning in Sichuan Province, Yibin 644000, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

以岩桂内生真菌交链孢霉（Alternaria）LJX27为研究对象，通过单因素试验研究各发酵工艺因素对黄樟素产量的影响，在此基础上通过二次通用旋转组合设计和响应面法优化黄樟素发酵工艺参数。结果表明：交链孢霉LJX27在初始pH 8、发酵温度 24 ℃、转速200 r/min、培养5 d的条件下，黄樟素产量最高，达2.13 g/L，比优化前黄樟素的产量提高了67.72%。各因素对黄樟素产量的影响大小为初始pH值＞发酵时间＞发酵温度＞转速。该回归模型可以用于优化黄樟素的发酵条件。

关键词: 二次通用旋转组合设计, 岩桂, 黄樟素, 发酵条件

Abstract:

The objective of this study was to optimize fermentation conditions of Alternaria LJX27 (an endophytic fungus
from Cinnamomum pauciflorum) for enhanced production of safrole by response surface methodology with quadratic general
rotary composite design. The production of safrole was investigated with respect to fermentation conditions including
inoculum amount, pH, fermentation temperature, fermentation time and shaking speed. Fermentation experiments performed
under the optimal conditions: medium pH 8; temperature, 24 ℃; shaking speed, 200 r/min; and incubation time, 5 d; resulted
in maximum production of 2.13 g/L, which was 67.72% higher than that obtained before optimization. Safrole production
was affected in decreasing order by initial medium pH, fermentation time, fermentation temperature and shaking speed. Our
investigations indicated that the developed regression model was applicable for optimizing the fermentation conditions for
safrole production.

Key words: quadratic general rotary composite design, Cinnamomum pauciflorum, safrole, fermentation condition

中图分类号:

TS201.3

徐洲，尹礼国，张超，魏琴. 产黄樟素内生真菌交链孢霉LJX27的发酵条件优化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417032.

XU Zhou, YIN Li-guo, ZHANG Chao, WEI Qin. Optimization of Fermentation Conditions of Alternaria LJX27 for Safrole Production[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417032.

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产黄樟素内生真菌交链孢霉LJX27的发酵条件优化

Optimization of Fermentation Conditions of Alternaria LJX27 for Safrole Production

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