食品科学

• 生物工程 • 上一篇    下一篇

甘肃河西走廊产区酿酒葡萄产毒霉菌的分离与鉴定

宋 蕤,韩舜愈,李 敏,王 婧,杨学山   

  1. 1.甘肃农业大学食品科学与工程学院,甘肃 兰州 730070;2.甘肃省葡萄与葡萄酒工程学重点实验室,甘肃 兰州 730070;
    3.甘肃省葡萄酒产业技术研发中心,甘肃 兰州 730070;4.甘肃农业大学生命科学技术学院,甘肃 兰州 730070
  • 出版日期:2014-10-15 发布日期:2014-10-17

Isolation and Identification of Mycotoxin-Producing Fungi in Wine Grapes from Hexi Corridor in Gansu

SONG Rui, HAN Shun-yu, LI Min, WANG Jing, YANG Xue-shan   

  1. 1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
    2. Grape and Wine Engineering Laboratory of Gansu Province, Lanzhou 730070, China;
    3. Wine Industry Technology Development Center of Gansu Province, Lanzhou 730070, China;
    4. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2014-10-15 Published:2014-10-17

摘要:

目的:了解甘肃河西走廊酿酒葡萄产区污染霉菌的主要类群,分析酿酒葡萄潜在的霉菌毒素污染。方法:采集河西走廊不同地区的酿酒葡萄,进行霉菌的分离纯化和鉴定,并通过高效液相色谱法测定霉菌代谢产物。结果:鉴定出4 株污染酿酒葡萄的优势菌,分别为青霉属的黄绿青霉(Penicillium decumbens)和扩展青霉(Penicillium expansum),曲霉属的杂色曲霉(Aspergullus versicolor)和烟曲霉(Aspergillus fumigatus);扩展青霉能够产生展青霉毒素。结论:根据污染酿酒葡萄霉菌的情况,可确定河西走廊酿酒葡萄产区存在展青霉毒素污染的风险。

关键词: 甘肃河西走廊, 酿酒葡萄, 产毒霉菌, 展青霉毒素

Abstract:

Objective: To understand the dominant groups of contaminant fungi and analyze the potential fungal contaminants
on wine grapes from Hexi Corridor in Gansu. Methods: A primary investigation was conducted on wine grapes from
different regions in Hexi Corridor. Samples were separated and identified. The toxin-producing ability of the isolated strains
was analyzed by high performance liquid chromatography (HPLC). Results: Penicillium decumbens, Penicillium expansum,
Aspergullus versicolor, and Aspergillus fumigatus were identified as the dominant contaminating fungi on wine grapes.
Penicillium expansum was found to be able to produce patulin. Conclusion: Based on the fungal contamination of wine
grapes, the grape-growing areas of Hexi Corridor are at risk of patulin contamination.

Key words: Hexi Corridor in Gansu, wine grape, mycotoxin-producing fungi, patulin

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