食品科学 ›› 2021, Vol. 42 ›› Issue (3): 30-37.doi: 10.7506/spkx1002-6630-20200226-283

• 基础研究 • 上一篇    下一篇

不同整形方式对‘赤霞珠’葡萄果皮非花色苷酚的影响

刘美迎,迟明,张振文   

  1. (1.西北农林科技大学葡萄酒学院,陕西 杨凌 712100;2.潍坊学院,山东 潍坊 261061;3.青海省轻工业研究所有限责任公司,青海 西宁 810000;4.陕西省葡萄与葡萄酒工程技术研究中心,陕西 杨凌 712100)
  • 发布日期:2021-02-25
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2019YFD1002500)

Analysis of Non-anthocyanin Phenolics in ‘Cabernet Sauvignon’ (Vitis vinifera L.) under Different Training Systems

LIU Meiying, CHI Ming, ZHANG Zhenwen   

  1. (1. College of Enology, Northwest A & F University, Yangling 712100, China; 2. Weifang University, Weifang 261061, China; 3. Qinghai Light Industry Institute Co. Ltd., Xining 810000, China; 4. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China)
  • Published:2021-02-25

摘要: 以欧亚种酿酒葡萄‘赤霞珠’为试材,通过高效液气相色谱-质谱联用法研究单干单臂、单干双臂和单干双层双臂3 种整形方式对葡萄果皮非花色苷酚类物质的影响,并采用聚类分析和偏最小二乘判别分析(partial least squares-discrimination analysis,PLS-DA)筛选不同整形方式间单体非花色苷酚的差异组分。结果表明:与单干单臂和单干双层双臂整形相比,单干双臂整形可以提高葡萄果皮中的槲皮素-3-O-葡萄糖醛酸苷、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-半乳糖苷、槲皮素-3-O-鼠李糖苷类黄酮醇物质的含量;单干单臂和单干双臂整形葡萄果皮中杨梅酮-3-O-葡萄糖苷、异鼠李亭-3-O-葡萄糖苷、丁香亭-3-O-葡萄糖苷类黄酮醇类物质和表没食子酸儿茶素、儿茶素类黄烷醇类物质及原儿茶酸类、香草酸酚酸类物质的含量均高于或显著高于单干双层双臂整形,而单干双层双臂整形葡萄果皮中仅原花色素C1的含量较高。聚类分析结果显示,部分单体非花色苷酚类物质受年份的影响较大,且单干单臂和单干双臂整形对非花色苷酚类物质的影响模式类似,而两者与单干双层双臂整形区别较大。PLS-DA结果表明,不同整形方式的葡萄果皮中单体非花色苷酚类物质组分差异较大。槲皮素-3-O-葡萄糖苷、杨梅酮-3-O-葡萄糖苷等黄酮醇类物质是3 类整形方式所处理的葡萄果皮的主要差异组分,且对单干双臂整形处理组的贡献值较大,单干双臂整形的葡萄果皮中含有较高的黄酮醇类物质含量。综上,单干双臂整形方式有助于葡萄果皮非花色苷酚类物质的积累,可被应用于陕西关中地区酿酒葡萄的栽培生产中。

关键词: 整形方式;酿酒葡萄;‘赤霞珠’;非花色苷酚

Abstract: Cabernet Sauvignon (Vitis vinifera L.) grape berries were used to investigate the influences of three training systems, single guyot (SG), spur-pruned vertical shoot-positioned (VSP), and four-arm kniffin (4AK) , on non-anthocyanin phenolics, and then the clustering analysis and partial least squares-discriminant analysis (PLS-DA) were conducted to evaluate the characteristic non-anthocyanin phenolic constituents from each training system. The results showed that compared to SG and 4AK, VSP resulted in higher contents of quercetin-3-O-glucuronide, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, quercetin-3-O-rhamnoside in grape skins. Besides, the contents of flavonols including myricetin-3-O-glucoside, isorhametin-3-O-glucoside and syringetin-3-O-glucoside, flavan-3-ols including gallocatechin and catechin as well as protocatechuic acid and vanillic acid in grape skins were higher in SG and VSP than in 4AK, while only procyanin C1 was more abundant in 4AK. The clustering analysis showed that some non-anthocyanin phenolics were significantly affected by vintages, and SG and VSP impacted non-anthocyanin phenolics similarly to each other, but quite differently from 4AK. PLS-DA results showed significant differences in the contents of individual non-anthocyanins among training systems. Flavonols such as quercetin-3-O-glucosid and myricetin-3-O-glucoside were the differential non-anthocyanin phenolic components among training systems, which were found to be more important contributors to VSP and more abundant. In summary, VSP training system plays a positive role in the accumulation of non-anthocyanin phenolics, and can be used in wine grape cultivation in the Guanzhong region of Shaanxi.

Key words: training systems; wine grape; ‘Cabernet Sauvignon’; non-anthocyanin phenolics

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