食品科学

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Trichothecium roseum蛋白激发子诱导杏果抗黑斑病的作用机理

余晓燕,毕 阳,李永才*,燕 璐,沈科萍,胡林刚,唐亚梅   

  1. 甘肃农业大学食品科学与工程学院,甘肃 兰州 730070
  • 出版日期:2015-02-25 发布日期:2015-02-16
  • 通讯作者: 李永才
  • 基金资助:

    甘肃省农牧厅生物技术专项(GNSW-2011-11)

Mechanism of Alternaria Rot Resistance Induced in Apricot Fruits by Protein Elicitor from Trichothecium roseum

YU Xiaoyan, BI Yang, LI Yongcai*, YAN Lu, SHEN Keping, HU Lingang, TANG Yamei   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2015-02-25 Published:2015-02-16
  • Contact: LI Yongcai

摘要:

研究非亲和菌株粉红单端孢(Trichothecium roseum)菌体蛋白激发子对杏果实抗黑斑病的诱导效果及其作用机理。以‘兰州大接杏’为试材,通过处理后不同时间损伤接种杏果黑斑病菌(Alternaria alternata),研究T. roseum蛋白激发子对杏果实黑斑病抗病性的诱导及其时效性,同时进一步研究最佳处理质量浓度对杏果实组织活性氧及抗性相关酶的影响。结果表明,T. roseum蛋白激发子处理可显著抑制损伤接种杏果病斑的扩展(P<0.05),其中14.4 μg/mL蛋白激发子的控制效果最好,且诱导处理72 h时杏果产生最大抗性。T. roseum蛋白激发子处理后杏果组织O2-·的生成速率和H2O2含量分别在48 h和72 h出现明显的峰值,同时处理的果实组织超氧化物歧化酶、过氧化物酶、多酚氧化酶、β-1,3-葡聚糖酶和几丁质酶活性均有所增强。T. roseum菌体蛋白激发子处理能显著增强杏果组织的抗性,其可能是通过激发活性氧爆发和提高杏果实抗病性相关酶的活性来实现的。可见T. roseum蛋白激发子可以作为一种有潜力的诱抗剂应用于采后病害的控制。

关键词: 粉红单端孢菌, 蛋白激发子, 杏果实, 活性氧, 诱导抗病性, 黑斑病

Abstract:

The effect of protein elicitor from the nonhost Trichothecium roseum on black rot of apricot fruits and its possible
induction mechanisms were studied. The inductive effect and its time-dependent manner of the protein elicitor in apricot
fruits inoculated with Alternaria alternate at different times after treatment were studied. The effect of selected optimum
concentration of protein elicitor on reactive oxygen species (ROS) and resistance-related enzymes in apricot fruit tissue
were also evaluated. The lesion development of wound-inoculated apricot fruits were significantly reduced by protein elicitor
treatment(P < 0.05), and at 72 h after treatment with 14.4 μg/mL protein elicitorthe best induction effect on apricot fruits was
observed. The rate of superoxide anion (O2
- ·) production and the content of hydrogen peroxide (H2O2) in fruit tissue reached the
peak at 48 and 72 h, respectively. The activities of superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO),
β-1,3-glucanase (GLU) and chitinase (CHT) in apricot fruit tissue treated with the protein elicitor were also increased. The protein
elicitor treatment could significantly increase the disease resistance of apricot fruit tissue, and the possible mechanism might be due
to the outburst of ROS and the improved activities of resistance-related enzymes. So protein elicitor from Trichothecium roseum
may be a potential resistance inducer and can be further applied to postharvest diseases control.

Key words: Trichothecium roseum, protein elicitor, apricot fruit, reactive oxygen species, induced disease resistance, Alternaria rot

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