响应面优化罗耳阿太菌胞外多糖提取工艺及其保湿、黏度特性分析

doi:10.7506/spkx1002-6630-201508012

食品科学

响应面优化罗耳阿太菌胞外多糖提取工艺及其保湿、黏度特性分析

魏明1，李鸿梅1,*，马艳秋2，赵慧1，张桂弘1，闵伟红1

1.吉林农业大学食品科学与工程学院，吉林长春 130118；2.吉林农业大学图书馆，吉林长春 130118

出版日期:2015-04-25 发布日期:2015-04-28
通讯作者: 李鸿梅
基金资助:
吉林省科技发展计划项目（20126037）

Extraction and Viscosity Stability of Exopolysaccharides with Moisture-Retention Capacity from Athelia rolfsii

WEI Ming1, LI Hongmei1,*, MA Yanqiu2, ZHAO Hui1, ZHANG Guihong1, MIN Weihong1

1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;
2. Jilin Agricultural University Library, Changchun 130118, China

Online:2015-04-25 Published:2015-04-28
Contact: LI Hongmei

摘要/Abstract

摘要：

利用响应面分析法优化乙醇醇沉提取罗耳阿太菌胞外多糖（Athelia rolfsii exopolysaccharides，AEPS）工艺，并探究其保湿特性及黏度稳定性。以AEPS提取量为指标，在单因素试验的基础上，应用Box-Behnken试验设计优化多糖提取工艺。以壳聚糖和尿素作对照，研究AEPS吸湿及保湿性能，同时研究温度、pH值和离子（Mg2＋、Ca2＋、Na＋、K＋）浓度对AEPS黏度稳定性的影响。结果表明：AEPS醇沉最佳条件为浓缩倍数3、醇沉时间17 h、醇沉温度5 ℃、无水乙醇体积为发酵液的1.7 倍，所得AEPS提取量为12.24 g/L；AEPS吸湿及保湿性能明显优于壳聚糖和尿素，1 mg/mL AEPS（黏度57 mPa·s）保湿性最好；在5～95 ℃范围内，35 ℃时AEPS黏度最低，35 ℃以外多糖黏度都高于51 mPa·s，但在pH 1～12、离子浓度0～1.0 mol/L范围内，AEPS黏度几乎不受影响。

关键词: 罗耳阿太菌胞外多糖, 醇沉, 吸湿性, 保湿性, 黏度稳定性

Abstract:

The conditions of ethanol precipitation for Athelia rolfsii exopolysaccharides (AEPS) were optimized by response
surface methodology. Moisture-retention capacity and viscosity stability of AEPS were also determined. Using AEPS yield
as the response variable, optimal ethanol precipitation process for AEPS was determined by Box-Behnken design based on
single factor tests. Compared to chitosan and urea, the moisture-absorption and moisture-retention capacities of AEPS were
also studied. In addition, the effects of temperature, pH and ion concentration (Mg2+, Ca2+, Na+, and K+) on the viscosity
stability of AEPS were examined. The optimal conditions of ethanol precipitation for AEPS were determined as follows: 3-fold
condensation of the fermentation supernatant before 17 h precipitation at 5 ℃ with an ethanol/fermentation supernatant
ratio of 1.7:1 (V/V). Under these conditions, the AEPS yield was 12.24 g/L. The moisture-absorption and moisture-retention
capacities of AEPS were superior to those of chitosan and urea. The optimal concentration for moisture-retention was
1 mg/mL (57 mPa·s). At temperatures in the range of 5–95 ℃, the lowest viscosity of AEPS was 51 mPa·s at 35 ℃.
However, AEPS viscosity was very stable in the pH range of 1 to 12 and at ion concentrations ranging from 0 to 1.0 mol/L.

Key words: Athelia rolfsii exopolysaccharides (AEPS), ethanol precipitation, moisture-absorption capacity, moisture-retention capacity, viscosity stability

中图分类号:

TS201.7

魏明1，李鸿梅1,*，马艳秋2，赵慧1，张桂弘1，闵伟红1. 响应面优化罗耳阿太菌胞外多糖提取工艺及其保湿、黏度特性分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201508012.

WEI Ming1, LI Hongmei1,*, MA Yanqiu2, ZHAO Hui1, ZHANG Guihong1, MIN Weihong1. Extraction and Viscosity Stability of Exopolysaccharides with Moisture-Retention Capacity from Athelia rolfsii[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201508012.

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响应面优化罗耳阿太菌胞外多糖提取工艺及其保湿、黏度特性分析

Extraction and Viscosity Stability of Exopolysaccharides with Moisture-Retention Capacity from Athelia rolfsii

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