食品科学

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正交试验优化黄河鲤鱼鳞酶促溶性胶原蛋白提取工艺

肖 枫1,2,朱文学1,*,康怀彬1,刘丽莉1,贺家亮1,任国艳1   

  1. 1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.江苏大学食品与生物工程学院,江苏 镇江 212013
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 通讯作者: 朱文学

Orthogonal Array Design for the Optimization of Pepsin Soluble Collagen Extraction from Cyprinus carpio haematopterus Scale

XIAO Feng1,2, ZHU Wenxue1,*, KANG Huaibin1, LIU Lili1, HE Jialiang1, REN Guoyan1   

  1. 1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China;
    2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
  • Online:2015-06-25 Published:2015-06-12
  • Contact: ZHU Wenxue

摘要:

为提高黄河鲤鱼鳞胶原蛋白提取率,在4 ℃条件下,采用胃蛋白酶和0.5 mol/L乙酸溶液对其提取工艺进行研究,在单因素试验的基础上,采用正交试验设计方法探索了提取时间、加酶量和料液比3 个因素对黄河鲤鱼鳞酶促溶性胶原蛋白(pepsin soluble collagen,PSC)提取率的影响。结果表明,黄河鲤鱼鳞PSC的最适提取工艺参数为提取时间60 h、加酶量40 kU/g和料液比1∶10(g/mL),在此条件下,PSC提取率达到(17.7±0.7)%。所提取的产品与酸溶性胶原蛋白(acid soluble collagen,ASC)有相似的电泳性质和氨基酸组成,表明其可能与ASC具有相似的分子结构。

关键词: 黄河鲤鱼鳞, 胶原蛋白, 提取工艺

Abstract:

Pepsin and 0.5 mol/L acetic acid were used to enhance the extraction of collagen from Cyprinus carpio
haematopterus scale at 4 ℃. Using combination of single factor experiments and orthogonal array design, the effect of
extraction time, enzyme concentration and ratio of solid to solvent on the yield of pepsin soluble collagen (PSC) from
C. haematopterus scale were explored. The results showed that the optimal conditions for PSC extraction were determined
as follows: extraction time, 60 h; enzyme concentration, 40 kU/g; and ratio of solid to solvent, 1:10 (g/mL). The yield of
PSC under these conditions was (17.7 ± 0.7)%. The PSC had electrophoretic properties amino acid composition similar to
those of acid soluble collagen (ASC), suggesting similarity beween their molecular structures.

Key words: Cyprinus carpio haematopterus scale, collagen, extraction process

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