超声处理对鲫鱼皮胶原蛋白的自组装行为和理化性质影响

doi:10.7506/spkx1002-6630-20190826-275

食品科学 ›› 2020, Vol. 41 ›› Issue (19): 98-104.doi: 10.7506/spkx1002-6630-20190826-275

超声处理对鲫鱼皮胶原蛋白的自组装行为和理化性质影响

贾俊强，孙晟源，周晓瑞，缪楠，朱玉杰，吴琼英

（1.江苏科技大学粮食学院，江苏?镇江 212004；2.江苏科技大学生物技术学院，江苏?镇江 212018）

出版日期:2020-10-15 发布日期:2020-10-23
基金资助:
国家自然科学基金青年科学基金项目（31401641）

Effect of Ultrasonic Treatment on Self-Assembly Behavior and Physicochemical Properties of Collagen from Carassius auratus Skin

JIA Junqiang, SUN Shengyuan, ZHOU Xiaorui, MIAO Nan, ZHU Yujie, WU Qiongying

(1. School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China; 2. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China)

Online:2020-10-15 Published:2020-10-23

摘要/Abstract

摘要： 为研究超声处理对鲫鱼皮胶原纤维形成和理化性质的影响，本实验比较了超声处理前后胶原蛋白的自组装动力学、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱、荧光光谱、圆二色光谱、纤维形态和NIH/3T3细胞增殖能力。结果表明：超声功率100～200 W、超声时间1～10 min时，鲫鱼皮胶原蛋白的自组装成核期明显缩短，纤维生成率增加；当超声功率为600 W或超声时间大于10 min时，鲫鱼皮胶原蛋白的亚基开始降解，胶原I型特征弱化；超声处理后，胶原纤维的正负Cotton效应比值呈现下降趋势，荧光吸收强度明显增加，发射波长蓝移约3 nm，胶原蛋白疏松多孔，其纤维形态均匀；细胞增殖实验结果表明，在超声功率100～200 W和超声时间1～10 min的范围内，超声波处理能够显著改善鲫鱼皮胶原蛋白的NIH/3T3细胞增殖能力（P＜0.05）；在200 W处理1 min后可使鲫鱼皮胶原蛋白的NIH/3T3细胞增殖活性提高10.1%。因此，适度超声处理能够促进鲫鱼皮胶原蛋白的自组装进程，有效改善其结构及理化特性，为鲫鱼皮胶原蛋白改性提供一种新的方法。

关键词: 超声处理；鲫鱼；胶原蛋白；自组装；理化性质

Abstract: In order to determine the effect of ultrasonic treatment on the fibril formation and physicochemical properties of collagen from Carassius auratus skin, the self-assembly kinetics, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pattern, fluorescence spectra, circular dichroism spectra, fibrillar morphology and NIH/3T3 cell proliferation ability of the collagens with and without ultrasonic treatment were analyzed and compared. The results showed that the nucleation phase of collagen self-assembly was significantly shortened and the fibril formation rate was increased after ultrasonic treatment at 100–200 W for 1–10 min. When the ultrasonic power was 600 W or the ultrasonic time was longer than 10 min, the collagen subunit was degraded, and its characteristics as type I collagen were weakened. After sonication, the ratio of positive to negative Cotton effect (RPN) of collagen from Carassius auratus skin showed a downward trend, and its fluorescence intensity was increased remarkably, with about 3 nm blue shift being observed in the maximum emission wavelength. In addition, the collagen showed a large number of pores and loose cross-sections and its fibers were uniform in morphology after ultrasonic treatment. The results of cell proliferation test showed that ultrasonic treatment in the power range of 100–200 W for 1–10 min could significantly improve the NIH/3T3 cell proliferation capacity of collagen from Carassius auratus skin (P < 0.05). After ultrasonic treatment at 200 W for 1 min, the NIH/3T3 cell proliferation capacity of the collagen was increased by 10.1%. In conclusion, moderate sonication can promote the self-assembly process of collagen from Carassius auratus skin, and effectively improve its structure and physicochemical properties. This study suggests that ultrasonic treatment is an effective modification method for collagen from Carassius auratus skin.

Key words: ultrasonic treatment; Carassius auratus; collagen; self-assembly; physicochemical properties

中图分类号:

TS254.9

贾俊强，孙晟源，周晓瑞，缪楠，朱玉杰，吴琼英. 超声处理对鲫鱼皮胶原蛋白的自组装行为和理化性质影响[J]. 食品科学, 2020, 41(19): 98-104.

JIA Junqiang, SUN Shengyuan, ZHOU Xiaorui, MIAO Nan, ZHU Yujie, WU Qiongying. Effect of Ultrasonic Treatment on Self-Assembly Behavior and Physicochemical Properties of Collagen from Carassius auratus Skin[J]. FOOD SCIENCE, 2020, 41(19): 98-104.

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超声处理对鲫鱼皮胶原蛋白的自组装行为和理化性质影响

Effect of Ultrasonic Treatment on Self-Assembly Behavior and Physicochemical Properties of Collagen from Carassius auratus Skin

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