食品科学

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外源乙烯对桃果实采后贮藏过程中线粒体内细胞色素氧化酶的影响

阚 娟,张庆庆,万 冰,刘 俊,金昌海*   

  1. 扬州大学食品科学与工程学院,江苏 扬州 225127
  • 出版日期:2015-12-25 发布日期:2015-12-24
  • 通讯作者: 金昌海
  • 基金资助:

    国家自然科学基金青年科学基金项目(31101586);江苏省科技计划项目(BC2013401);
    江苏省自然科学基金项目(BK2010310)

Effect of Exogenous Ethylene on Mitochondrial Cytochrome C Oxidase during Postharvest Storage of Peach Fruit

KAN Juan, ZHANG Qingqing, WAN Bing, LIU Jun, JIN Changhai   

  1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China  
  • Online:2015-12-25 Published:2015-12-24
  • Contact: JIN Changhai

摘要:

以硬溶质型桃果实‘加纳岩’为试材,研究外源乙烯处理对桃果实采后贮藏过程中呼吸速率、乙烯释放量、细胞色素氧化酶(cytochrome C oxidase,COX)活性以及线粒体编码的COX 3 种亚基(COXⅠ 、COXⅡ 、COXⅢ )基因表达量的影响。结果表明:外源乙烯处理加速了硬溶质型桃果实‘加纳岩’在采后贮藏过程中硬度的下降,促进了呼吸作用和内源乙烯释放,使呼吸高峰和乙烯释放高峰提前出现。同时外源乙烯抑制了COX酶活性,抑制了贮藏后期COXⅠ 和COXⅡ 亚基基因的表达,抑制了整个贮藏过程中COXⅢ 亚基基因的表达。外源乙烯处理抑制了COX途径的呼吸,加速了果实的软化衰老。

关键词: 桃, 乙烯, 线粒体, 呼吸速率, 细胞色素氧化酶

Abstract:

The effect of exogenous ethylene on respiration rate, ethylene production, cytochrome C oxidase (COX) activity
and the gene expression levels of mitochondrial DNA-encoded subunits Ⅰ, Ⅱ and Ⅲ of cytochrome C oxidase in fruits of
the stony-hard peach (Prumus persica) cultivar ‘Jianayan’ was determined during postharvest storage. The results showed
that exogenous ethylene accelerated the decline of firmness and promoted the respiration rate and ethylene production of
peach fruits during postharvest storage and resulted in earlier appearance of the peaks of respiration and ethylene production.
Meanwhile, exogenous ethylene inhibited COX activity and the expression level of COX Ⅲ gene throughout the storage
process and suppressed the expression of COXⅠ and COXⅡ genes in the later stage of the storage period. Exogenous
ethylene inhibited the respiration via COX pathway and accelerated the softening and senescence of fruit.

Key words: peach, ethylene, mitochondria, respiration rate, cytochrome C oxidase

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