食品科学

• 生物工程 • 上一篇    下一篇

酶法制取大豆油脂过程中的蛋白酶解动力学

张 辉1,齐宝坤2,李 杨2,隋晓楠2,王中江2,江连洲2,*   

  1. 1.科技部中国农村技术开发中心,北京 100045;2.东北农业大学食品学院,黑龙江 哈尔滨 150030
  • 出版日期:2016-01-15 发布日期:2016-01-15

Kinetics of Protein Hydrolysis during Aqueous Enzymatic Extraction of Soybean Oil

ZHANG Hui1, QI Baokun2, LI Yang2, SUI Xiaonan2, WANG Zhongjiang2, JIANG Lianzhou2,*   

  1. 1. China Rural Technology Development Center, Ministry of Science and Technology of China, Beijing 100045, China;
    2. College of Food Science, Northeast Agricultural University, Harbin 150030, China
  • Online:2016-01-15 Published:2016-01-15

摘要:

通过数学方法推导和对Alcalase碱性蛋白酶酶解大豆中蛋白实验的系统研究,得到Alcalase碱性蛋白酶酶解大豆中蛋白的动力学模型为:R=(18.294 0E0+0.273 4ρ0)exp(-0.256 2DH),式中:E0为初始蛋白酶质量浓度,ρ0为初始底物质量浓度,DH为水解度。通过数学推导和对大豆蛋白酶解反应过程中Alcalase碱性蛋白酶失活的系统研究,得到膨化大豆蛋白的酶解反应过程中Alcalase碱性蛋白酶失活的动力学常数K=4.920 4 min-1。通过拟合实验证明,建立的动力学模型与实验结果具有较好的拟合效果,证明所建立的动力学模型具有较高的实际应用价值。

关键词: 大豆, 酶法制油, 蛋白酶解, 酶解动力学

Abstract:

The hydrolysis of extruded full-fat soybean flakes by alkaline protease provides a promising method to extract
soybean oil, protein and polypeptide simultaneously. In order to control the enzyme-assisted aqueous extraction of oil from
full-fat soybean flakes, it is necessary to model the kinetics of the protein hydrolysis of extruded full-fat soybean flakes
and establish the relevant kinetic parameters. In this research, we developed a kinetic mathematical model for the alcalase
hydrolysis of full-fat soybean flakes as follows: R =(18.294 0E0 + 0.273 4ρ0) exp(-0.256 2DH), where E0 is the initial
concentration of alcalase, ρ0 is initial substrate concentration, and DH is degree of hydrolysis. The kinetic constant (K) of the
inactivation of alcalase during the hydrolysis process was determined to be 4.920 4 min-1. Our experimental data were well
fitted to the proposed kinetic model, proving that the mode has a high practical value.

Key words: soybean, enzyme-assisted aqueous extraction of soybean oil, protein hydrolysis, enzymatic hydrolysis kinetics

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