关键词: 葡萄枝蔓, 原花色素, 铁盐催化比色法, 香草醛法, 直接紫外分光光度法

Abstract:

The contents of proanthocyanidins in grape vines from 13 different varieties were analyzed by four different
methods, namely ferric ion catalysis-colorimetry, H2SO4-vanillin assay, HCl-vanillin assay and UV spectrophotometry.
The quantitative determination by UV spectrophotometry was carried out using 3 different proanthocyanidin reference
substances. The methodological evaluation and statistical analysis showed that ferric ion catalysis-colorimetry was timeconsuming
and vanillin assays had poor stability, though both methods had a high specificity. On the other hand, the
UV spectrophotometric method was more stable, precise and convenient. For all 13 grape varieties tested, there was an
approximately 2-fold difference in proanthocyanidin contents in grape vines assayed by UV spectrophotometry using 3
different reference substances. As a result, the purity and composition of reference substances had a significant influence on
the results of proanthocyanidins determination.

Key words: grape vine, proanthocyanidins, ferric ion catalysis-colorimetry, vanillin assay, UV spectrophotometry