灵芝孢子粉破壁工艺优化及其抗肿瘤作用

doi:10.7506/spkx1002-6630-201614009

摘要/Abstract

摘要：

以灵芝孢子粉为原料，采用高压均质法破壁灵芝孢子，研究灵芝孢子粉破壁优化工艺及破壁孢子粉抗肿瘤作用。选取水为破壁溶剂，以均质压力、均质次数、料液比为试验因素，以灵芝孢子破壁率为试验指标，通过单因素试验及正交试验对灵芝孢子破壁工艺进行优化。通过建立S180荷瘤小鼠模型，对破壁灵芝孢子粉的抗肿瘤作用进行研究。结果表明，最佳破壁工艺条件为均质压力150 MPa、均质次数3、料液比1∶100（g/mL），破壁率为94.35%。抗肿瘤实验表明，破壁灵芝孢子对小鼠S180肉瘤的抑制率为43.37%～57.59%，且肝体比和肺体比变化稳定，并可显著提高小鼠的胸腺指数和脾指数。因此，破壁灵芝孢子粉具有良好的抗肿瘤作用。

关键词: 灵芝孢子, 破壁, 高压均质法, 抗肿瘤作用

Abstract:

The purpose of this study was to investigate the optimum conditions for breaking Ganoderma lucidum spores
by high-pressure homogenization using water as the solvent and to evaluate the antitumor effect of the broken spores.
The optimization of three process variables including solid-to-liquid ratio, homogenization pressure, and the number
of homogenization cycles for improved percentage of broken spores was conducted through one-factor-at-a-time and
orthogonal array experiments. S180 tumor-bearing mice models were established for evaluation of the antitumor effect of
the broken G. lucidum spores. The results showed that the optimal conditions for breaking G. lucidum spores were obtained
as follows: homogenization pressure, 150 MPa; homogenization cycles, 3 times; and solid-to-liquid ratio, 1:100 (g/mL).
Under these conditions, the percentage of broken spores could be up to 94.35%. Antitumor tests showed that the percentage
inhibition of tumor growth in S180 tumor-bearing mice by the broken spores was 43.37%–57.59%, and the liver index and
lung index were little affected while the thymus index and spleen index were significantly enhanced. Therefore, broken G.
lucidum spores possessed good antitumor effect.

Key words: Ganoderma lucidum spores, spore disruption, high pressure homogenization method, antitumor effect

中图分类号:

R285.5

刘春延，张国财，程方志，张国珍，赵博，林连男. 灵芝孢子粉破壁工艺优化及其抗肿瘤作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201614009.

LIU Chunyan, ZHANG Guocai, CHENG Fangzhi, ZHANG Guozhen, ZHAO Bo, LIN Liannan. Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614009.

参考文献

[1] 姜余梅, 杨艳, 陈晓姗, 等. 灵芝孢子和抗性淀粉对糖尿病大鼠糖脂

代谢及氧化应激的协同干预[J]. 食品科学, 2014, 35(23): 288-291.

DOI:10.7506/spkx1002-6630-201423056.

[2] LIU Xin, YUAN Jianping, CHUNG C K, et al. Antitumor activity

of the sporoderm-broken germinating spores of Ganoderma

lucidum[J]. Cancer Lett, 2002, 182(2): 155-161. DOI:10.1016/S0304-

3835(02)00080-0.

[3] GUO Liang, XIE Jianhui, RUAN Yuanyuan, et al. Characterization

and immunostimulatory activity of a polysaccharide from the spores

of Ganoderma lucidum[J]. International Immunopharmacology, 2009,

9(10): 1175-1182. DOI:10.1016/j.intimp.2009.06.005.

[4] JIN Hai, JIN Feng, JIN Jiaxing, et al. Protective effects of Ganoderma

lucidum spore on cadmium hepatotoxicity in mice[J]. Food and

Chemical Toxicology, 2013, 52(1): 171-175. DOI:10.1016/

j.fct.2012.05.040.

[5] ZHOU Yan, QUA Zeqiang, ZENG Yuanshan, et al. Neuroprotective

effect of preadministration with Ganoderma lucidum spore on rat

hippocampus[J]. Experimental and Toxicologic Pathology, 2012,

64(7/8): 673-680. DOI:10.1016/j.etp.2010.12.011.

[6] 王淑秋, 李晓捷, 姜志梅, 等. 灵芝孢子粉对癫痫大鼠脑组织细胞

色素C、线粒体钙、HSP70和BDNF的影响[J]. 中国病理生理杂志,

2011, 27(6): 1053-1058. DOI:10.3969 /j.issn.1000-4718.2011.06.003.

[7] WANG Quanxi, HUANG Yifan, WU Baocheng, et al. Inhibitive effect

on apoptosis in splenic lymphocytes of mice pretreated with Lingzhi

(Ganoderma lucidum) spores[J]. Journal of Traditional Chinese Medicine,

2014, 34(2): 173-177. DOI:10.1016/S0254-6272(14)60074-1.

[8] 唐柳, 张志军, 魏雪生, 等. 灵芝孢子粉药理作用研究进

展[ J ] . 天津农业科学, 2 0 1 1 , 1 7 ( 3 ) : 2 5 - 2 8 . D O I : 1 0 . 3 9 6 9 /

j.issn.1006-6500.2011.03.008.

[9] 黄晓兰, 吴惠勤, 黄芳, 等. 破壁与不破壁灵芝孢子粉多糖的分析[J].

中草药, 2006, 37(6): 813-816.

[10] S A N O D I Y A B S , T H A K U R R K , B A G H E L G B , e t a l .

Ganoderma lucidum: a potent pharmacological macrofungus[J].

Current Pharmaceutical Biotechnology, 2009, 10(8): 717-742.

DOI:10.2174/138920109789978757.

[11] ZHU H S, YANG X L, WANG L B, et al. Effects of extracts from

sporoderm-broken spores of Ganoderma lucidum on HeLa cells[J].

Cell Biology and Toxicology, 2000, 16(3): 201-206. DOI:10.1023/

A:1007663006548.

[12] FU Yujie, LIU Wie, ZU Yugang, et al. Breaking the spores of the

fungus Ganoderma lucidum by supercritical CO2[J]. Food Chemistry,

2009, 112(1): 71-76. DOI:10.1016/j.foodchem.2008.05.044.

[13] 赵洁胜, 魏联, 虞燕萍, 等. 灵芝孢子粉破壁率的检验方法研究进

展[J]. 中国医药指南, 2013, 11(5): 431-434. DOI:10.15912/j.cnki.

gocm.2013.05.071.

[14] 朱俊洁, 张守勤, 王长征, 等. 破壁提取综合法提取灵芝孢子多糖的

研究[J]. 农业机械学报, 2004, 35(3): 184-185.

[15] 倪伟锋. 灵芝破壁孢子粉破壁率测定技术和质量安全评价的研究[D].

福州: 福建农林大学, 2008.

[16] 焦艳丽, 温升南, 杜冰, 等. 挤压处理灵芝孢子粉提取灵芝多糖[J].

食品科学, 2011, 32(16): 67-70.

[17] 张秀娟, 刘斌, 孙宇婷, 等. 大豆多糖对S-(180)荷瘤小鼠抗肿瘤

作用的免疫机制研究[J]. 食品工业科技, 2012, 33(2): 389-392.

DOI:10.13386/j.issn1002-0306.2012.02.010.

[18] 张艳, 叶克难. 虫草灵芝孢子粉复合物对小鼠免疫功能的影响[J].

食品科学, 2012, 33(17): 269-273.

[19] 杨丽丽, 邓志钦, 黄维渊, 等. 灵芝孢子粉对抗亚急性酒精性肝损伤

的实验研究[J]. 时珍国医国药, 2013, 24(3): 513-514. DOI:10.3969/

j.issn.1008-0805.2013.03.001.

[20] 张荣标, 陈润, 陈冠敏, 等. 破壁灵芝孢子粉对小鼠免疫功能影响的

研究[J]. 预防医学论坛, 2013, 19(12): 936-938; 954.

[21] 金玲, 刘菊妍, 孙升云, 等. 灵芝孢子油软胶囊对H22肝癌小鼠抑瘤

作用及免疫功能的影响[J]. 中华中医药杂志, 2011, 26(4): 715-718.

[22] 蒋丽, 黄远英, 殷光玲. 灵芝孢子油免疫调节作用的研究[J]. 现代食

品科技, 2013, 29(3): 531-533; 542.

[23] 李洁. 白花蛇舌草对S-(180)荷瘤小鼠抑瘤作用的实验研究[J]. 中医

研究, 2008, 21(5): 11-13.

[24] 吴学谦, 吴学良, 楼利明, 等. 原木灵芝破壁孢子粉对小鼠免疫

功能的影响[J]. 中国食用菌, 2014, 33(1): 51-54. DOI:10.13629/

j.cnki.53-1054.2014.01.017.

[25] 马晶晶. 高速离心剪切粉碎对灵芝孢子粉破壁及特性的影响[D].

武汉: 武汉理工大学, 2006.

[26] 林庶茹, 杜松, 郑洪新, 等. 灵芝破壁孢子粉对LEWIS荷瘤小鼠细

胞免疫干预作用[J]. 中国中医基础医学杂志, 2005, 11(6): 428-

430; 433.