食品科学

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罗非鱼皮胶原蛋白水解产物的体外抗氧化活性和体内抗衰老作用

周先艳,樊 建,唐远龙,庄永亮,孙丽平*   

  1. 昆明理工大学 云南省食品安全研究院,云南 昆明 650500
  • 出版日期:2016-08-15 发布日期:2016-08-30

In vitro Antioxidant Activity and in vivo Anti-Aging Effect of Tilapia Skin Gelatin Hydrolysates

ZHOU Xianyan, FAN Jian, TANG Yuanlong, ZHUANG Yongliang, SUN Liping*   

  1. Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 650500, China
  • Online:2016-08-15 Published:2016-08-30

摘要:

对罗非鱼皮胶原蛋白水解制备水解产物(tilapia skin gelatin hydrolysate,TSGH),分离得到3 个肽组分(TSGH-1、TSGH-2和TSGH-3),分析肽组分氨基酸组成,并对TSGH-3的体外抗氧化活性和体内抗衰老能力进行评价。结果表明:TSGH-3的分子质量低,疏水性氨基酸含量高,从自由基清除和金属螯合两个方面证实了TSGH-3具有很好的体外抗氧化活性。TSGH-3能显著增加由D-半乳糖诱导衰老大鼠的血清、肝脏和肾脏组织的总抗氧化能力、超氧化物歧化酶和谷胱甘肽过氧化物酶活性,并且能显著降低丙二醛的含量。此外,TSGH-3的摄入可以保护大鼠的肝脏和肾脏指数。因此,TSGH-3在抗氧化、抗衰老方面具有较好的应用潜力。

关键词: 罗非鱼皮胶原蛋白水解产物, 氨基酸组成, 抗氧化活性, 抗衰老

Abstract:

The amino acid composition of three peptides fractions (TSGH-1, TSGH-2 and TSGH-3) separated from tilapia
skin gelatin hydrolysate (TSGH) was measured. Additionally, the antioxidant activity in vitro and anti-aging capacity
in vivo of TSGH-3 were evaluated. The results showed TSGH-3 had low molecular weight and high hydrophobic amino
acid contents. TSGH-3 had high antioxidant activity in vitro in terms of free radical scavenging and metal chelating
activity. Furthermore, the in vivo anti-aging activity in a D-galactose-induced aging mouse model indicated that TSGH-3
could significantly increase T-AOC, SOD and GSH-Px activities in serum, liver and kidney tissues of rats (P < 0.05), and
significantly decreased MDA contents (P < 0.05). Besides, liver and kidney indices of rats could be also protected after the
intake of TSGH-3. Therefore, TSGH-3 possesses high antioxidant activity and can be used as a natural anti-aging agent in
the medicinal and food industries.

Key words: tilapia skin gelatin hydrolysates, amino acid composition, antioxidant activity, anti-aging

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