食品科学

• 生物工程 • 上一篇    下一篇

米曲霉Aspergillus oryzae M-4降解己烯雌酚的特性研究

胡凯弟1,邓维琴1,陈树平1,柴先杜1,刘爱平1,卓文杰1,刘书亮1,2,*   

  1. 1.四川农业大学食品学院,四川 雅安 625014;2.四川农业大学,食品加工与安全研究所,四川 雅安 625014
  • 出版日期:2016-09-15 发布日期:2016-09-22

Degradation Characteristics of Diethylstilbestrol by Aspergillus oryzae M-4

HU Kaidi1, DENG Weiqin1, CHEN Shuping1, CHAI Xiandu1, LIU Aiping1, ZHUO Wenjie1, LIU Shuliang1,2,*   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
    2. Research Institute of Food Processing and Security, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2016-09-15 Published:2016-09-22

摘要:

以1 株分离自酱油曲的米曲霉(Asperg i l lus oryzae)M-4为材料,初步研究其降解己烯雌酚(diethylstilbestrol,DES)的特性。米曲霉M-4对DES的降解率与菌体生物量呈正相关,在基础盐培养基(mineralsalt medium,MM)中培养9 d对100 mg/L的DES降解率为93%。动力学研究表明,该菌株降解DES的过程符合一级动力学方程,在所测试的培养温度、初始pH值、底物质量浓度范围内,DES半衰期为1.645~5.295 d。培养温度30 ℃和偏酸性环境有利于其对DES的降解;底物质量浓度越高,其半衰期越长。

关键词: 己烯雌酚, 降解, 米曲霉, 特性

Abstract:

Aspergillus oryzae M-4, isolated from soy sauce koji, was applied in the present study to investigate its
degradation characteristics for diethylstilbestrol (DES). The degradation rate of DES had a positive correlation with the
biomass of Aspergillus oryzae M-4 and reached up to 93% when the strain was cultured in MM medium with 100 mg/L DES
for 9 d. Kinetic studies indicated that the process of degradation fitted well with a first-order kinetic equation. The half-life
of DES was 1.645–5.295 d under the tested conditions of culture temperature, initial pH and substrate concentration. Culture
temperature of 30 ℃ and acidic environment were favorable for the degradation of DES, while high concentration of DES
extended its half-life.

Key words: diethylstilbestrol (DES), degradation, Aspergillus oryzae, characteristics

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