利用不同疏水色谱柱分离蜂王浆主蛋白1

doi:10.7506/spkx1002-6630-201701020

食品科学

利用不同疏水色谱柱分离蜂王浆主蛋白1

汪雪玉1，乔江涛1，刘印康1,2，张红城1,3，董捷1,3,*

1.中国农业科学院蜜蜂研究所，北京 100093；2.哈尔滨商业大学食品学院，黑龙江哈尔滨 150076；
3.农业部农产品加工中心蜂产品加工分中心，北京 100093

出版日期:2017-01-15 发布日期:2017-01-16

Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns

WANG Xueyu1, QIAO Jiangtao1, LIU Yinkang1,2, ZHANG Hongcheng1,3, DONG Jie1,3,*

1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
2. College of Food Science, Harbin University of Commerce, Harbin 150076, China;
3. National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China

Online:2017-01-15 Published:2017-01-16

摘要/Abstract

摘要： 目的：比较不同疏水色谱柱对蜂王浆主蛋白1（major royal jelly protein 1，MRJP 1）的分离效果，并筛选出适合分离MRJP 1寡聚体和单体的色谱柱。方法：首先分别采用12 种疏水色谱柱对蜂王浆蛋白粗提液进行分离，然后使用非变性电泳验证所分离MRJP 1组分的寡聚体和单体形式。结果：Hitrap Butyl HP疏水色谱柱可以将所有的MRJP 1从蜂王浆蛋白粗提液中分离出来，并得到分别以MRJP 1寡聚体和MRJP 1单体为主的2 个组分。结论：利用疏水色谱柱可以有效地分离MRJP 1。

关键词: 蜂王浆主蛋白1, 疏水色谱柱, 非变性电泳, 蛋白分离

Abstract: To make a comparison of different hydrophobic chromatography columns for separating major royal
jelly protein 1 (MRJP 1), and choose a suitable one to separate MRJP 1 oligomer and MRJP 1 monomer. Methods: Totally 12
kinds of hydrophobic chromatography columns were applied to separate crude royal jelly protein, then the MRJP 1 oligomer
and MRJP 1 monomer fractions were verified by native gel electrophoresis. Results: MRJP 1 was completely isolated by
hydrophobic interaction chromatography on Hitrap Butyl HP, and an MRJP 1 oligomer fraction and an MRJP 1 monomer
fraction were obtained. Conclusion: Hydrophobic chromatographic column provides an effective approach to separate
MRJP 1 from royal jelly.

Key words: major royal jelly protein 1, hydrophobic chromatography column, native gel electrophoresis, protein separation

中图分类号:

TS201.2

汪雪玉，乔江涛，刘印康，张红城，董捷. 利用不同疏水色谱柱分离蜂王浆主蛋白1[J]. 食品科学, doi: 10.7506/spkx1002-6630-201701020.

WANG Xueyu, QIAO Jiangtao, LIU Yinkang, ZHANG Hongcheng, DONG Jie. Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201701020.

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利用不同疏水色谱柱分离蜂王浆主蛋白1

Separation of Major Royal Jelly Protein 1 Using Different Hydrophobic Chromatographic Columns

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