基于FTIR和1H-NMR方法分析高压脉冲电场技术对抗氧化肽KWFH的结构影响

doi:10.7506/spkx1002-6630-201701019

食品科学

基于FTIR和1H-NMR方法分析高压脉冲电场技术对抗氧化肽KWFH的结构影响

王莹1,2，邢杰2，李幸芳2，殷涌光1,2,*

1.吉林大学生物与农业工程学院，吉林长春 130025；2.吉林大学食品科学与工程学院，吉林长春 130062

出版日期:2017-01-15 发布日期:2017-01-16

Using FTIR and 1H-NMR to Explore the Structure of Antioxidant Peptide KWFH Treated by Pulsed Electric Field (PEF)

WANG Ying1,2, XING Jie2, LI Xingfang2, YIN Yongguang1,2,*

1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China;
2. School of Food Science and Engineering, Jilin University, Changchun 130062, China

Online:2017-01-15 Published:2017-01-16

摘要/Abstract

摘要： 为了探究高压脉冲电场（pulsed electric field，PEF）提高抗氧化肽活性的机制，以抗氧化肽KWFH为实验材料，以1,1-二苯基-2-三硝基苯肼为衡量指标，通过双因素试验方案，考察电场强度和电场频率对其活性的影响。经过频率2 400 Hz、电场强度10 kV/cm的PEF处理，抗氧化肽KWFH活性提高了13.92%（P＜0.05）。借助傅里叶变换红外光谱（Fourier transform infrared spectroscopy，FTIR）、核磁共振氢谱（nuclear magnetic resonance，1H-NMR）、Zeta电位及圆二色谱技术，分析高压脉冲电场技术对其结构的影响。FTIR分析结果显示，经过PEF处理的样品羧酸羰基C＝O和苯环吸收峰强度增强；经过1H-NMR分析，推测羧酸羰基C＝O和苯环吸收强度变化引起官能团携带的氢质子变化。同时，经过PEF处理抗氧化肽KWFH的Zeta电位提高了8.70 mV（P＜0.05），进一步推测经过PEF处理，抗氧化肽KWFH结构更加无序，致使官能团暴露，吸收峰强度发生变化，引起肽活性改变。研究发现PEF处理对二级结构之间转化没有影响。这些研究为进一步探究PEF技术提高抗氧化肽活性机理提供了理论依据。

关键词: 高压脉冲电场, 抗氧化肽, 傅里叶变换红外光谱, 核磁共振

Abstract: This study attempted to explore the mechanism of the effect of pulsed electric field (PEF) on improving the
activity of antioxidant peptide. The effect of two PEF factors, electric field strength and pulse frequency, on the antioxidant
activity of KWFH, a commercial antioxidant peptide, was investigated by measuring its DPPH radical scavenging capacity.
The results showed that PEF treatment at a pulse frequency of 2 400 Hz and an electric field strength of 10 kV/cm resulted in
a 13.92% increase in the antioxidant activity of KWFH (P < 0.05). Fourier transform infrared spectroscopy (FTIR), nuclear
magnetic resonance (NMR), zeta potential and circular dichroism spectroscopy (CD) were used to analyze the structure
of the PEF-treated peptide. The FTIR analysis indicated that the absorption peak intensity of C＝O and aromatic ring in
the sample was enhanced. The 1H-NMR results led to a speculation that functional groups changes resulted in changes in
proton absorption intensity. At the same time, the Zeta potential value of the PEF-treated sample was increased by 8.70 mV
(P < 0.05). It was further speculated that after PEF treatment, the structure of the antioxidant peptide KWFH became
unordered, resulting in exposure of functional groups and an increase of absorption peak intensity, and eventually changes
in peptide activity. Moreover, PEF treatment had no effect on the transformation of the secondary structure. This study can
provide a theoretical basis for improving the activity of antioxidant peptides by PEF treatment, and for further exploring the
underlying mechanism.

Key words: pulsed electric field (PEF), antioxidant peptide, Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR)

中图分类号:

TS201.2

王莹，邢杰，李幸芳，殷涌光. 基于FTIR和1H-NMR方法分析高压脉冲电场技术对抗氧化肽KWFH的结构影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201701019.

WANG Ying, XING Jie, LI Xingfang, YIN Yongguang. Using FTIR and 1H-NMR to Explore the Structure of Antioxidant Peptide KWFH Treated by Pulsed Electric Field (PEF)[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201701019.

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基于FTIR和1H-NMR方法分析高压脉冲电场技术对抗氧化肽KWFH的结构影响

Using FTIR and 1H-NMR to Explore the Structure of Antioxidant Peptide KWFH Treated by Pulsed Electric Field (PEF)

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