食品科学 ›› 2019, Vol. 40 ›› Issue (1): 196-201.doi: 10.7506/spkx1002-6630-20170926-390

• 营养卫生 • 上一篇    下一篇

大学生饮食习惯与唾液微生物多样性的关联

张国庆,黄子琪,王明月,孔俊豪,李余动*,陈建设   

  1. 浙江工商大学食品与生物工程学院,浙江 杭州 310018
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    浙江省自然科学基金项目(LY14C060001)

Association between Dietary Habits and Salivary Microbial Diversity in College Students

ZHANG Guoqing, HUANG Ziqi, WANG Mingyue, KONG Junhao, LI Yudong*, CHEN Jianshe   

  1. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:通过16S rRNA基因高通量测序分析,研究不同饮食习惯大学生的唾液菌群多样性。方法:在大学生群体中通过问卷调查的方式,选取36 位受试者,其中19 位同学喜荤且饮食以荤食为主(A组),17 位同学喜素且饮食以素食为主(B组)。采取唾液样本,提取DNA,聚合酶链式反应扩增,利用Illumina测序平台对16S rRNA V3~V4区进行双端测序,利用QIIME等软件进行细菌群落结构及多样性分析。结果:共获得990 286 条优质序列,检测出212 个可操作分类单元,归属于16 个门、143 个属。A组和B组唾液微生物群落多样性分析显示,在门水平上,A组中SR1、Fusobacteria、Candidatus、Saccharibacteria的丰度显著低于B组。在属水平上,A组中的优势属,如消化链球菌(Peptostreptococcus)、微单胞菌(Parvimonas)、优杆菌(Eubacterium)、Saccharibacteria_genera_incertae_sedis、SR1_genera_incertae_sedis、卡氏菌(Catonella)、艰难杆菌(Mogibacterium)、Solobacterium相对丰度均比B组显著降低(P<0.05),而A组中棒状杆菌属(Corynebacterium)相对丰度比B组显著升高(P<0.05)。结论:人类口腔唾液有复杂的微生物群落结构,本研究表明大学生人群的饮食习惯对其口腔微生物的群落结构可能有显著影响。

关键词: 饮食习惯, 微生物组, 群落多样性, 16S rRNA高通量测序, QIIME

Abstract: Objective: To study the salivary bacterial diversity of college students with different dietary habits through high throughput sequencing analysis of 16S rRNA gene. Methods: A total of 36 subjects were selected for a questionnaire survey including 19 students with a preference for meat having a meat-dominated diet (group A), and 17 students with a preference for vegetables having a vegetable-dominated diet (group B). Saliva samples were collected to extract DNA, followed by polymerase chain reaction (PCR) amplification and high-throughput sequencing of bacterial 16S rRNA V3–V4 region using the Illumina platform. The bacterial community structure and diversity were analyzed using the QIIME software. Results: A total of 990 286 high-quality reads were obtained and clustered into 212 operational taxonomic units (OTUs) for further analysis. A total of 16 bacterial phyla and 143 genera were detected. The diversity analysis of salivary microbiota showed that at the phylum level, SR1, Fusobacteria, Candidatus and Saccharibacteria were significantly decreased in group A as compared to group B; at the genus level, the relative abundance of the dominant genera Peptostreptococcus, Parvimonas, Eubacterium, Saccharibacteria_genera_incertae_sedis, SR1_genera_incertae_sedis, Catonella, Mogibacterium, and Solobacterium in group A were significantly lower than in group B, while the opposite was observed for the genus Corynebacterium (P<0.05). Conclusion: Human saliva has a complex microbial community structure. Dietary habits in college students (meat eaters and vegetarians) may have a significant influence on the structure of the oral microbial community.

Key words: dietary habits, microbiome, bacterial diversity, high throughput sequencing of 16S rRNA gene, QIIME

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