食品科学 ›› 2017, Vol. 38 ›› Issue (23): 191-195.doi: 10.7506/spkx1002-6630-201723030

• 营养卫生 • 上一篇    下一篇

猪皮胶原多肽螯合钙增加大鼠骨密度

许先猛1,2,董文宾1,*   

  1. 1.陕西科技大学化学与化工学院,陕西 西安 710021;2.运城职业技术学院有机食品工程系,山西 运城 044000
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    “十二五”国家科技支撑计划项目(2012BAD12B07-05)

Calcium-Binding Collagen Polypeptide from Pigskin Gelatin Improves Bone Mineral Density in Rats

XU Xianmeng1,2, DONG Wenbin1,*   

  1. 1. College of Chemistry and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China; 2. Department of Organic Food Engineering, Yuncheng Polytechnic College, Yuncheng 044000, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 以实验室制备的猪皮胶原多肽(collagen polypeptide,CP)和葡萄糖酸钙为原料,采用液体反应法合成胶原多 肽螯合钙(calcium-binding CP,CP-Ca),并评价CP-Ca增加SD大鼠骨密度(bone mineral density,BMD)作用。将120 只 断乳雌性4 周龄SD大鼠随机分为10 组:1)对照组(灌胃去离子水);2)低、中、高剂量CP-Ca组(139.28、278.55、 557.10 mg/(100 g·d));3)低、中、高剂量CaCO3组(25、50、100 mg/(100 g·d));4)低、中、高 剂量CP+CaCO3组(139.28 mg/(100 g·d)CP+25 mg/(100 g·d)CaCO3、278.55 mg/(100 g·d)CP+ 50 mg/(100 g·d)CaCO3、557.10 mg/(100 g·d)CP+100 mg/(100 g·d)CaCO3)。所有实验大鼠饲喂基础饲 料,自由饮用去离子水。实验过程中,每周测定大鼠的体质量和身长,灌胃第4周做3 d钙代谢实验。测定大鼠血清 钙(S-Ca)、血清磷(S-P)浓度、碱性磷酸酶(alkaline phosphatese,ALP)活力、大鼠BMD和骨钙含量,并对 大鼠股骨进行扫描电子显微镜(scanning electron microscope,SEM)分析。结果显示,中、高剂量CP-Ca组和高剂 量CP+CaCO3组可以显著促进大鼠体质量增加和发育(P<0.05),CP-Ca效果更为明显。CP-Ca组和CP+CaCO3组 的ALP活力显著低于对照组(P<0.05),而中、高剂量CP-Ca组和中、高剂量CP+CaCO3组BMD显著高于对照组 (P<0.05),且高剂量CP-Ca组高于对应剂量CP+CaCO3组,各剂量CP-Ca组和CP+CaCO3组钙表观吸收率分别高 于对应CaCO3组,CP-Ca具有促进大鼠骨生长、发育、骨钙沉积的作用。SEM结果显示,CP-Ca和CP+CaCO3能够 预防和改善大鼠因长期钙摄入不足引起的骨质疏松症。CP-Ca溶解性高、解离性好,富含钙元素及多肽,具有预防 骨质疏松症、增加骨质密度的作用。

关键词: 猪皮明胶, 多肽螯合钙, SD大鼠, 骨密度

Abstract: In this paper, calcium-binding collagen polypeptide (CP-Ca) was synthesized by liquid-state reaction between collagen polypeptide prepared in our laboratory from pigskin gelatin and calcium gluconate, and the effect of CP-Ca on improving bone mineral density (BMD) in rats was investigated. Totally 120 female SD rats were equally divided into 10 groups: control group (deionized water by gavage), low-, middle- and high-dosage CP-Ca groups (139.28, 278.55 and 557.10 mg/(100 g·d)), low-, middle- and high-dosage CaCO3 groups (25, 50 and 100 mg/(100 g·d)), and low-, middle- and high-dosage CP + CaCO3 group (139.28 mg /(100 g·d) CP + 25 mg/(100 g·d) CaCO3, 278.55 mg/(100 g·d) CP + 50 mg/(100 g·d) CaCO3, 557.10 mg/(100 g·d) CP + 100 mg/(100 g·d) CaCO3). All rats were fed on a basic diet and deionized water. The body weight and length of the rats were recorded weekly. A three-day calcium metabolism experiment was carried out in the fourth week to estimate the apparent absorptivity of calcium. Serum calcium (SCa), serum phosphorusand (S-P), and alkaline phosphatese (ALP), BMD and bone calcium content were measured. The rat femurs were analyzed by scanning electron microscope (SEM). The results showed that CP-Ca at middle and high dosages and CP + CaCO3 at high dosage could significantly promote body weight gain (P < 0.05), and the effect of CPCa was more apparent. CP-Ca and CP + CaCO3 had obviously lower ALP activity than the control group (P < 0.05). CP-Ca and CP + CaCO3 both at middle and high dosages significantly raised BMD compared to the control group (P < 0.05), and CP-Ca was more effective at the high dosage. At all dosages tested, CP-Ca and CP + CaCO3 resulted in higher Ca bioavailability than CaCO3. CP-Ca could activate osteoblasts on the surface of bones, increase the density of bones and reduce bone calcium loss. Meanwhile, SEM revealed that CP-Ca and CP + CaCO3 could prevent and improve osteoporosis caused by long-term calcium deficiency. CP-Ca was highly soluble, exhibited good dissociation ability, and was rich in calcium and polypeptides so than it can be used in functional foods and health products to prevent osteoporosis and improve BMD.

Key words: pigskin gelatin, calcium-binding collagen polypeptide (CP-Ca), SD rats, bone mineral density (BMD)

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