食品科学 ›› 2017, Vol. 38 ›› Issue (23): 207-212.doi: 10.7506/spkx1002-6630-201723033

• 营养卫生 • 上一篇    下一篇

菠萝蜜多糖对脾淋巴细胞抗氧化作用及免疫功能的影响

朱科学1,王颖倩1,2,张彦军1,贺书珍1,徐 飞1,吴 刚1,谭乐和1,*   

  1. 1.中国热带农业科学院香料饮料研究所,海南 万宁 571533;2.黑龙江八一农垦大学工程学院,黑龙江 大庆 163319
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    海南省自然科学基金面上项目(317270);南昌大学食品科学与技术国家重点实验室开放基金项目(SKLF-KF-201602)

Antioxidant and Immunoenhancing Activity of Polysaccharide from Artocarpus heterophyllus Lam. on Spleen Lymphocytes

ZHU Kexue1, WANG Yingqian1,2, ZHANG Yanjun1, HE Shuzhen1, XU Fei1, WU Gang1, TAN Lehe1,*   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China;2. College of Engineering, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 本研究旨在探明菠萝蜜多糖(polysaccharide from jackfruit pulp,JFP-Ps)对正常小鼠脾淋巴细胞增殖和 免疫调节的影响。通过体外小鼠脾淋巴细胞的培养实验,测定不同质量浓度JFP-Ps对正常小鼠脾淋巴细胞增殖、 抗氧化能力(总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力和丙二醛 (malonic dialdehyde,MDA)含量)及细胞因子(肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ (interferon-γ,IFN-γ)和白细胞介素-1β(interleukin-1β,IL-1β))分泌水平的影响。结果显示,JFP-Ps可促进小 鼠脾淋巴细胞增殖,质量浓度为80 μg/mL和160 μg/mL时效果显著(P<0.05);当JFP-Ps质量浓度为40 μg/mL甚至 更高时,T-AOC及GSH-Px、SOD和CAT活力显著升高(P<0.05),但MDA含量显著降低(P<0.05);JFP-Ps在质 量浓度40~160 μg/mL范围内可显著促进脾淋巴细胞TNF-α、IFN-γ和IL-1β的分泌(P<0.05)。结果表明JFP-Ps的免疫 增强活性可能与其促进小鼠脾淋巴细胞增殖、提高抗氧化活性及诱导细胞因子TNF-α、IFN-γ和IL-1β分泌水平有关。

关键词: 菠萝蜜多糖, 脾淋巴细胞, 抗氧化活性, 细胞因子, 免疫调节

Abstract: The antioxidant and immunomodulatory effects of water-soluble polysaccharide from jackfruit (Artocarpus heterophyllus Lam.) pulp (JFP-Ps) were investigated. The effect of JFP-Ps on the proliferation of normal mouse spleen lymphocytes, antioxidant capacity (total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activity) and malonic dialdehyde (MDA) contents, as well as the secretion of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) were detected by culturing mouse spleen lymphocytes in vitro in the presence of different concentrations of JFP-Ps. The results showed that JFP-Ps at 80 and 160 μg/mL significantly (P < 0.05) increased lymphocyte proliferation, and it at 40 μg/mL and above enhanced T-AOC, GSH-Px, SOD and CAT activities and reduced MDA content. Moreover, JFP-Ps at 40, 80 and 160 μg/mL significantly increased the secretion of TNF-α, IFN-γ and IL-1β in spleen lymphocytes (P < 0.05). These results indicate that JFP-Ps exerts immunomodulatory effect likely by inducing lymphocyte proliferation, enhancing antioxidant activity and increasing the secretion of TNF-α, IFN-γ and IL-1β.

Key words: polysaccharide from jackfruit (Artocarpus heterophyllus Lam.), spleen lymphocyte, antioxidant activity, cytokines, immune modulation

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