食品科学 ›› 2019, Vol. 40 ›› Issue (10): 257-264.doi: 10.7506/spkx1002-6630-20180515-223

• 成分分析 • 上一篇    下一篇

紫甘蓝矢车菊色素苷的分析及其苷元的制备

蒋晓岚1,石渝凤1,付周平1,成 琳2,刘亚军2,高丽萍2,夏 涛1,*   

  1. 1.安徽农业大学茶与食品科技学院,安徽 合肥 230036;2.安徽农业大学生命科学学院,安徽 合肥 230036
  • 出版日期:2019-05-25 发布日期:2019-05-31
  • 基金资助:
    安徽农业大学稳定与引进人才项目(yj2017-37);安徽省自然科学基金项目(1708085QC51)

Analysis and Preparation of Cyanidin Glycosides from Purple Cabbage

JIANG Xiaolan1, SHI Yufeng1, FU Zhouping1, CHENG Lin2, LIU Yajun2, GAO Liping2, XIA Tao1,*   

  1. 1. School of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China; 2. School of Life Science, Anhui Agricultural University, Hefei 230036, China
  • Online:2019-05-25 Published:2019-05-31

摘要: 以紫甘蓝为原料,首先对紫甘蓝花青素苷进行定性分析,鉴定16 种矢车菊色素苷;然后对矢车菊色素苷的提取条件进行优化。结果表明,最佳提取条件为含1%盐酸的50%乙醇溶液、料液比1∶10(g/mL);利用AB-8型大孔树脂对矢车菊色素苷进行初步纯化后,通过高温酸水解的方法获得游离态矢车菊色素粗品;再利用C18柱层析的方法纯化得到矢车菊色素单体;最后通过结晶沉淀方法获得纯度大于99%的矢车菊色素单体粉末。

关键词: 紫甘蓝, 矢车菊色素, 分离纯化, 结晶

Abstract: In this experiment we identified 16 cyanidin glycosides from purple cabbage. Cyanidins were extracted from purple cabbage under the optimized conditions as follows: 50% ethanol containing 1% hydrochloric acid as extraction solvent, and a solid-to-solvent ratio of 1:10 (g/mL). The extract was purified with macroporous resin AB-8 and then hydrolyzed with acid at high temperature to free cyanidin glycosides. Furthermore, the hydrolysate was purified by C18 column chromatography, yielding cyanidin monomers with 99% purity.

Key words: purple cabbage, cyanidin, extraction and purification, crystallization

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