食品科学 ›› 2019, Vol. 40 ›› Issue (15): 338-345.doi: 10.7506/spkx1002-6630-20180702-025

• 专题论述 • 上一篇    下一篇

免疫磁分离技术在食源性致病菌快速检测中的研究进展

曹 潇,赵力超,陈 洵,谢 会,张竟丰,刘卓坤,王 丽   

  1. 1.华南农业大学食品学院,广东 广州 510642;2.暨南大学食品安全与营养研究院,广东 广州 510632;3.广州双螺旋基因技术有限公司,广东 广州 510006
  • 出版日期:2019-08-15 发布日期:2019-08-26
  • 基金资助:
    广东省科技计划项目(2016A040403103;2017B020207004);广东省农业标准化研究项目(2015、2016年度);广东省质量工程项目《食品微生物检验学》;广东大学生科技创新培育专项资金项目(pdjhb0085)

Progress in the Application of Immunomagnetic Separation in Rapid Detection of Foodborne Pathogens

CAO Xiao, ZHAO Lichao, CHEN Xun, XIE Hui, ZHANG Jingfeng, LIU Zhuokun, WANG Li   

  1. 1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Research Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, China; 3. Double Helix Gene Technology Company, Guangzhou 510006, China
  • Online:2019-08-15 Published:2019-08-26

摘要: 建立食源性致病菌快速高效的检测新方法和策略对控制食源性疾病的爆发至关重要。样品前处理是快速筛选病原体的关键步骤。常规食源性致病菌检测技术通常需要前增菌和选择性分离等预处理过程才能达到提高目标细菌浓度的目的,耗时长且灵敏度低。免疫磁分离技术(immunomagnetic separation,IMS)是一种能够在较短时间内从复杂食品样品中分离和浓缩目标病原菌的技术,已被应用于多种食源性致病菌的检测。本文综述了IMS在食源性致病菌检测中的应用,重点阐述了IMS作用原理、影响IMS效果的因素以及结合其他检测技术在食源性致病菌快速检测中的最新应用研究,以期为该技术更深层次的应用研究和我国食品安全快速检测技术的发展提供理论支持。

关键词: 免疫磁分离技术, 磁分离效果, 食源性致病菌, 样品前处理, 快速检测, 食品安全

Abstract: Establishing a new fast and efficient detection method and strategy for foodborne pathogens is critical to control the outbreak of foodborne diseases. Sample preparation is a crucial step in the quick screening of pathogens. Conventional techniques for the detection of foodborne pathogens usually require pre-enrichment and selective separation for increased concentrations of the target bacteria, which are time-consuming and have low sensitivity. Immunomagnetic separation (IMS) is capable of separating and concentrating the target pathogens from complex food samples in a short period of time, which has been applied to the detection of various foodborne pathogens. This article reviews the application of IMS in the detection of foodborne pathogens. Meanwhile, it mainly focuses on the principle of IMS and the factors influencing the efficiency of IMS, as well as the latest progress in its combined application with other detection technologies in rapid detection of foodborne pathogens. This review aims at providing theoretical support for deeper applications of IMS and the development of rapid food safety detection technologies in China.

Key words: immunomagnetic separation, immunomagnetic separation efficiency, foodborne pathogens, sample preparation, rapid detection, food safety

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