关键词: 猪血蛋白酶解物, 酒精性肝损伤, 抗氧化活性, 小鼠

Abstract: Objective: The protective effect of porcine blood hydrolysate (PBH) on acute alcoholic hepatic injury in mice was investigated. Methods: The mice were randomly divided into blank control, model control, reduced glutathione (20 mg/kg mb) and PBH (0.83, 1.70 and 3.33 g/kg mb) groups. All mice except those in the blank control group were administered for 30 days. On day 31, 50% alcohol was given at a dose of 12 mL/kg mb to establish the animal model of acute liver damage. At 16 h post-administration, sera were collected to determine the activities of alanine aminotransferase (ALT) and aspartate transaminase (AST). All mice were killed by cervical dislocation for the assay of antioxidant enzyme activities in liver, and the degree of hepatic injury was analyzed by histological examination. Results: The levels of AST and ALT in serum and malondialdehyde, tumor necrosis factor alpha and interleukin-6 in liver obviously decreased in the PBH treatment groups when compared with the model control group, while the content of glutathione, and the activities of superoxide dismutase and glutathione peroxidase in liver remarkably increased. The pathological changes such as fatty degeneration in liver were significantly alleviated in the PBH groups. Conclusion: PBH can protect the mouse liver from alcohol-induced injury, and the underlying mechanism may be related to anti-inflammatory and antioxidant activities.

Key words: porcine blood hydrolysate, alcoholic liver injury, antioxidant activities, mice