食品科学 ›› 2020, Vol. 41 ›› Issue (20): 307-313.doi: 10.7506/spkx1002-6630-20190612-127

• 安全检测 • 上一篇    下一篇

动物性食品中氟苯尼考检测的电化学免疫传感器构建

刘卫华,杨茜,刘敏轩,于文龙,王向红   

  1. (河北农业大学食品科技学院,河北 保定 071000)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0401101)

Development of an Electrochemical Immunosensor for Rapid Quantification of Florfenicol in Animal-Derived Foods

LIU Weihua, YANG Xi, LIU Minxuan, YU Wenlong, WANG Xianghong   

  1. (College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 构建一种基于石墨烯-壳聚糖复合修饰材料的电化学免疫传感器,用于检测动物性食品中的氟苯尼考残留。在1~1 000 ng/mL范围内,氟苯尼考质量浓度与电极峰值电流呈线性关系,方法的检出限(RSN=3)为0.08 ng/mL。免疫传感器的选择性、特异性和稳定性均良好。对猪肉、牛肉、鸡肉和鸡蛋等实际样品进行检测,10、100、1 000 μg/kg三个加标水平的回收率在76.90%~94.30%之间。经液相色谱-串联质谱法对电化学方法进行验证,两种方法的相关性较高。建立的免疫传感器方法准确可靠,可以应用于现场大批量样品的检测。

关键词: 兽药残留;氟苯尼考;电化学免疫传感器;石墨烯-壳聚糖

Abstract: An electrochemical immunosensor based on graphene-chitosan composites was established to detect florfenicol residues in animal-derived foods. A linear relationship between the peak current and the concentration of florfenicol was observed in the range of 1–1 000 ng/mL and the detection limit (RSN = 3) was 0.08 ng/mL. The immunosensor displayed good selectivity, specificity and stability. The average recoveries from pork, beef, chicken and egg samples spiked at concentration levels of 10, 100 and 1 000 μg/kg ranged from 76.90% to 94.30%. The reliability of the electrochemical method was confirmed by a high correlation between it and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established immunosensor method was accurate and reliable and could be applied to the on-site detection of massive samples.

Key words: veterinary drug residues; florfenicol; electrochemical immunosensor; graphene-chitosan composite

中图分类号: