石竹烯对热杀索丝菌的抑菌机理

doi:10.7506/spkx1002-6630-20190725-334

食品科学 ›› 2020, Vol. 41 ›› Issue (15): 31-38.doi: 10.7506/spkx1002-6630-20190725-334

石竹烯对热杀索丝菌的抑菌机理

舒慧珍，唐志凌，韩薇，陈海明，陈卫军，胡月英，陈文学

（1.海南大学食品科学与工程学院，海南海口 570228；2.海南大学材料与化工学院，海南海口 570228）

出版日期:2020-08-15 发布日期:2020-08-19
基金资助:
国家自然科学基金地区科学基金项目（31760480）；国家自然科学基金应急管理项目（31640061）

Antibacterial Activity and Mechanism of Caryophyllene against Brochothrix thermosphacta

SHU Huizhen, TANG Zhiling, HAN Wei, CHEN Haiming, CHEN Weijun, HU Yueying, CHEN Wenxue

(1. College of Food Science and Engineering, Hainan University, Haikou 570228, China;2. College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China)

Online:2020-08-15 Published:2020-08-19

摘要/Abstract

摘要： 本实验研究石竹烯对热杀索丝菌的抑菌效果及其作用机理。通过石竹烯对热杀索丝菌的最小抑菌浓度（minimum inhibition concentration，MIC）和生长曲线的测定考察其抑制活性；通过观察扫描电子显微镜和碱性磷酸酶的泄漏判定石竹烯对细胞形态和细胞壁结构的破坏程度；通过钾离子、二乙酸荧光素分子和蛋白质的泄漏考察其对细胞膜的影响；通过苹果酸脱氢酶以及丙酮酸激酶活力的测定探究其对细胞代谢的影响；通过荧光光谱扫描法研究石竹烯与细菌DNA的结合能力。结果表明，石竹烯对热杀索丝菌的MIC值为4.51 mg/mL，1×MIC和2×MIC石竹烯能够破坏细胞的形态、结构以及细胞膜的通透性，影响细胞代谢，导致苹果酸脱氢酶、丙酮酸激酶活力显著降低（P＜0.05），同时对基因组DNA构象和结构造成破坏。

关键词: 石竹烯, 热杀索丝菌, 抑菌机理, 苹果酸脱氢酶, 丙酮酸激酶, 基因组DNA

Abstract: In this paper, the antibacterial effect and mechanism of action caryophyllene against Brochothrix thermosphacta were studied. The inhibitory activity was investigated by determining the minimum inhibitory concentration (MIC) and growth curve. The degree of damage to cell morphology and cell wall structure caused by caryophyllene were determined respectively by using scanning electron microscopy (SEM) and measuring the leakage of alkaline phosphatase (AKP). The effect on the cell membrane was examined by measuring the leakage of potassium ions, fluorescein diacetate (FDA) and proteins. The effect on cell metabolism was investigated by measuring the activity of malate dehydrogenase (MDH) and pyruvate kinase (PK). The binding of caryophyllene to bacterial DNA was studied by fluorescence spectroscopy. The results showed that the MIC of caryophyllene against B. thermosphacta was 4.51 mg/mL. Caryophyllene at concentrations of 1 × MIC and 2 × MIC could destroy the morphology and structure of the cells and the permeability of the cell membrane, thus affecting cell metabolism, leading to a significant decrease in malate dehydrogenase and pyruvate kinase activities (P < 0.05), and destroying the conformation and structure of genomic DNA.

Key words: caryophyllene, Brochothrix thermosphacta, antibacterial mechanism, malate dehydrogenase, pyruvate kinase, genomic DNA

中图分类号:

TS201.3

舒慧珍, 唐志凌, 韩薇, 陈海明, 陈卫军, 胡月英, 陈文学. 石竹烯对热杀索丝菌的抑菌机理[J]. 食品科学, 2020, 41(15): 31-38.

SHU Huizhen, TANG Zhiling, HAN Wei, CHEN Haiming, CHEN Weijun, HU Yueying, CHEN Wenxue. Antibacterial Activity and Mechanism of Caryophyllene against Brochothrix thermosphacta[J]. FOOD SCIENCE, 2020, 41(15): 31-38.

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石竹烯对热杀索丝菌的抑菌机理

Antibacterial Activity and Mechanism of Caryophyllene against Brochothrix thermosphacta

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