食品科学 ›› 2020, Vol. 41 ›› Issue (20): 256-262.doi: 10.7506/spkx1002-6630-20190822-223

• 成分分析 • 上一篇    下一篇

超高效液相色谱-飞行时间-串联质谱法对3 种蓝靛果忍冬果实中花青苷的比较分析

张上上,郑姝宁,王学涛,倪阳,王英   

  1. (1.秦皇岛市食品药品检验中心,河北 秦皇岛 066000;2.中国农业科学院蔬菜花卉研究所,农业农村部蔬菜产品质量安全风险评估实验室(北京),北京 100081)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    秦皇岛市科技计划项目(201805A058)

Comparative Analysis of Anthocyanins in the Fruits of Three Varieties of Blue Honeysuckle (Lonicera caerulea L.) by Ultra-high Performance Liquid Chromatography-Time of Flight-Tandem Mass Spectrometry

ZHANG Shangshang, ZHENG Shuning, WANG Xuetao, NI Yang, WANG Ying   

  1. (1. Qinhuangdao Institute for Food and Drug Control, Qinhuangdao 066000, China;2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Risk Assessment Laboratory for Vegetable Product Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 利用超高效液相色谱-飞行时间-串联质谱技术,对伊利亚特、别列里和格尔达3 种蓝靛果忍冬果实中的花青苷进行分析。结果表明,在3 种蓝靛果忍冬果实中共鉴定出24 种花青苷,包含14 种矢车菊素类花青苷、6 种芍药素类花青苷、2 种飞燕草素类花青苷和2 种天竺葵素类花青苷,其中9 种花青苷为首次在蓝靛果忍冬中被发现。3 种蓝靛果忍冬果实中主要的花青苷均为矢车菊素-3-葡萄糖苷、芍药素-3-葡萄糖苷、矢车菊素-3-芸香糖苷和矢车菊素-3,5-双葡萄糖苷。比较分析表明,3 种蓝靛果忍冬果实中花青苷的组成不同,分别在伊利亚特、别列里和格尔达果实中检测到24、23 种和12 种花青苷;此外,伊利亚特和别列里果实中花青苷的相对含量明显高于格尔达。本实验为进一步研究花青苷在蓝靛果忍冬中合成与积累的分子遗传机制以及选育富含花青苷的蓝靛果忍冬新品种奠定基础。

关键词: 蓝靛果忍冬;花青苷;超高效液相色谱-飞行时间-串联质谱

Abstract: In this study, we performed a comparative analysis of anthocyanins in the fruits of three varieties of blue honeysuckle (Lonicera caerulea L.), namely Iliad, Berel and Gerda, using ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS). The results showed that a total of 24 anthocyanins were identified in all the samples, including 14 cyanidin anthocyanins, six peonidin anthocyanins, two delphinidin anthocyanins and two pelargonidin anthocyanins. Among them, nine anthocyanins were found in the fruits of blue honeysuckle for the first time. The main anthocyanins in the fruits of all three blue honeysuckle varieties were cyanidin-3-glucoside, peonidin-3-glucoside, cyanidin-3-rutinoside and cyanidin-3,5-diglucoside. Comparative studies showed that the composition of anthocyanins was different in the three varieties of blue honeysuckle. A total of 24, 23 and 12 anthocyanins were detected in the fruits of Iliad, Belegli and Gerda, respectively. In addition, the relative contents of anthocyanins in the fruits of Iliad and Berel were significantly higher than in Gerda. This study will provide the foundation for further study on the molecular genetic mechanism of anthocyanin synthesis and accumulation in blue honeysuckle and for the selection and breeding of new varieties of blue honeysuckle rich in anthocyanins.

Key words: blue honeysuckle (Lonicera caerulea L.); anthocyanins; ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry

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