食品科学 ›› 2020, Vol. 41 ›› Issue (17): 89-96.doi: 10.7506/spkx1002-6630-20190828-312

• 食品工程 • 上一篇    下一篇

辉光放电等离子体对硫色镰刀菌的杀菌作用

杜明远,龙海涛,田立鹏,付国瑞,毕阳,蒲陆梅   

  1. (1.甘肃农业大学理学院,甘肃 兰州 730070;2.甘肃农业大学食品科学与工程学院,甘肃 兰州 730070)
  • 出版日期:2020-09-15 发布日期:2020-09-16
  • 基金资助:
    国家自然科学基金地区科学基金项目(31860472)

Fungicidal Effect of Glow Discharge Plasma on Fusarium sulphureum

DU Mingyuan, LONG Haitao, TIAN Lipeng, FU Guorui, BI Yang, PU Lumei   

  1. (1. College of Science, Gansu Agricultural University, Lanzhou 730070, China; 2. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
  • Online:2020-09-15 Published:2020-09-16

摘要: 目的:研究辉光放电等离子体对硫色镰刀菌孢子的杀菌效果及活性影响。方法:以硫色镰刀菌标准菌株BNCC 117681为目标菌株,首先研究辉光放电等离子体在不同条件下对目标菌株的杀菌效果;随后设计Box-Behnken试验方案,考察电压、处理时间和极距及其相互作用对杀菌率的影响,并确定最佳杀菌工艺条件;最后研究辉光放电等离子体对该菌生长、细胞膜完整性及过氧化损伤程度的影响。结果:电压、处理时间和极距是显著影响杀菌率的因素,在电压560 V、处理时间15 min、电极极距1.5 cm时杀菌率最高,可达到92.73%。辉光放电等离子体处理该菌后,孢子萌发率、菌落直径、菌体干质量以及麦角甾醇质量浓度都显著减小(P<0.05);相对电导率、孢内大分子物质(蛋白质和核酸)渗漏量、碘化丙啶荧光染色强度以及丙二醛浓度都显著增大(P<0.05)。说明细胞膜很可能是等离子体作用于硫色镰刀菌的主要位点,从而造成细胞膜损伤及细胞死亡。结论:辉光放电等离子体对硫色镰刀菌孢子具有显著的杀灭效果,研究结果为该技术应用于食品中病原真菌杀灭提供了理论依据。

关键词: 辉光放电等离子体;硫色镰刀菌;活性;杀菌

Abstract: Objective: To study the fungicidal effect and influence of glow discharge plasma (GDP) on the spores of Fusarium sulphureum. Methods: The fungicidal effect of glow discharge plasma on the standard strain (BNCC 117681) of F. sulphureum was studied under different conditions. Then, Box-Behnken design was used to investigate the effects of voltage, time and inter-electrode distance as well as interactions among them on the fungicidal efficiency, and the optimum levels of these parameters were determined. Finally, the effects of glow discharge plasma on the growth, membrane integrity and peroxidative damage of the fungus were studied. The results showed that voltage, time and inter-electrode distance were the significant factors affecting the fungicidal efficiency. Under the conditions of voltage 560 V, time 15 min, and inter-electrode distance 1.5 cm, the highest fungicidal efficiency of 92.73% was obtained. After the strain was treated with glow discharge plasma, the spore germination rate, colony diameter, fungal dry mass and ergosterol concentration decreased significantly (P < 0.05); the relative conductivity, the leakage of macromolecular substances (protein and nucleic acid), the fluorescence staining intensity of propidium iodide and the concentration of malondialdehyde increased significantly (P < 0.05). The results indicated that the cell membrane was likely to be the main site for glow discharge plasma to attack F. sulphureum, causing damage to the cell membrane and consequently cell death. Conclusion: Glow discharge plasma has a significant fungicidal effect on F. sulphureum spores, which provides a theoretical basis for the application of this technique in the killing of pathogenic fungi in foods.

Key words: glow discharge plasma; Fusarium sulphureum; activity; fungicidal

中图分类号: