基于适配体杂交链式反应检测郫县豆瓣中黄曲霉毒素B1

doi:10.7506/spkx1002-6630-20190903-037

食品科学 ›› 2020, Vol. 41 ›› Issue (22): 301-307.doi: 10.7506/spkx1002-6630-20190903-037

基于适配体杂交链式反应检测郫县豆瓣中黄曲霉毒素B1

黄玉坤，陶璇，邵坤，王冲，王力均，车振明，陈祥贵

（1.西华大学食品与生物工程学院，四川成都 610039；2.宜宾西华大学研究院食品非热加工重点实验室，食品非热加工工程技术研究中心，四川宜宾 644004）

出版日期:2020-11-25 发布日期:2020-11-26
基金资助:
国家自然科学基金青年科学基金项目（31801647）；四川省科技厅重点研发项目（2018SZ0340；2019YFN0173；2020YFN0041）；西华大学省部级学科平台开放课题（szjj2016-022）；西华大学“青年学者后备人才”支持计划项目

Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer

HUANG Yukun, TAO Xuan, SHAO Kun, WANG Chong, WANG Lijun, CHE Zhenming, CHEN Xianggui

(1. School of Food and Biological Engineering, Xihua University, Chengdu 610039, China; 2. Key Laboratory of Food Non Thermal Processing, Engineering Technology Research Center of Food Non Thermal Processing, Yibin Xihua University Research Institute, Yibin 644004, China)

Online:2020-11-25 Published:2020-11-26

摘要/Abstract

摘要： 建立一种基于AFB1适配体杂交链式反应的荧光检测方法。研究cDNA和HP1、HP2浓度、杂交孵育时间、氧化石墨烯质量浓度对该方法性能的影响，确定最优条件为cDNA浓度50 nmol/L，HP1、HP2浓度60 nmol/L，杂交孵育时间60 min，氧化石墨烯质量浓度50 μg/mL。在该条件下，本方法的线性范围为2～60 ng/mL，检测限为1.84 ng/mL，在郫县豆瓣中的加标回收率为85.73%～94.25%。该方法用于12 种不同品牌的郫县豆瓣的检测，与国标中的酶联免疫吸附试剂盒检测结果无显著差异（P＞0.05）。本研究建立的检测方法能简单、快速、灵敏的检测郫县豆瓣中的AFB1含量，具有用于复杂基质样品中AFB1快速检测应用潜力。

关键词: AFB1；适配体；氧化石墨烯；杂交链式反应；郫县豆瓣

Abstract: A fluorescence method for the detection of aflatoxin B1 (AFB1) in Pixian broad-bean paste by using hybridization chain reaction (HCR) based on aptamer was established. The effects of the concentration of cDNA, HP1, HP2 and graphene oxide (GO), and incubation time for hybridization were studied on the performed of the method. The optimal conditions were obtained as follows: 50 nmol/L cDNA, 60 nmol/L HP1, 60 nmol/L HP2, 60 min incubation time, and 50 μg/mL GO. Under these conditions, the method showed a good linear relationship in the concentration range of 2–60 ng/mL, the detection limit was 1.84 ng/mL, and the recovery for spiked samples were 85.73%–94.25%. When the method was applied to the detection of 12 different brands of Pixian broad-bean pastes, satisfactory results were obtained which showed no significant difference from those of enzyme-linked immunosorbent assay in the national standard (P > 0.05). This method can detect AFB1 in various Pixian broad-bean pastes easily, quickly and sensitively, and has the potential for fast detection of AFB1 in complex samples.

Key words: aflatoxin B1; aptamer; graphene oxide; hybridization chain reaction; Pixian broad-bean paste

中图分类号:

TS207.3

黄玉坤，陶璇，邵坤，王冲，王力均，车振明，陈祥贵. 基于适配体杂交链式反应检测郫县豆瓣中黄曲霉毒素B1[J]. 食品科学, 2020, 41(22): 301-307.

HUANG Yukun, TAO Xuan, SHAO Kun, WANG Chong, WANG Lijun, CHE Zhenming, CHEN Xianggui. Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer[J]. FOOD SCIENCE, 2020, 41(22): 301-307.

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基于适配体杂交链式反应检测郫县豆瓣中黄曲霉毒素B1

Detection of Aflatoxin B1 in Pixian Broad-Bean Paste by Using Hybridization Chain Reaction Based on Aptamer

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