食品科学 ›› 2021, Vol. 42 ›› Issue (12): 328-333.doi: 10.7506/spkx1002-6630-20191128-278

• 安全检测 • 上一篇    

基质匹配曲线校正-HPLC-MS/MS法同时测定干性食品包装纸中8 种甜味剂

廖惠云,吴洋,马梦婕,毛淑蕊,朱怀远,陈晶波,张华,曹毅,朱龙杰,袁益来   

  1. (江苏中烟工业有限责任公司,江苏 南京 210019)
  • 出版日期:2021-06-25 发布日期:2021-06-29
  • 基金资助:
    江苏中烟工业有限责任公司科技创新项目(201911)

Simultaneous Determination of Eight Sweeteners in Dry Food Wrappings by Using Matrix Matched Solvent Calibration-High Performance Liquid Chromatography-Tandem Mass Spectrometry

LIAO Huiyun, WU Yang, MA Mengjie, MAO Shurui, ZHU Huaiyuan, CHEN Jingbo, ZHANG Hua, CAO Yi, ZHU Longjie, YUAN Yilai   

  1. (China Tobacco Jiangsu Industrial Co. Ltd., Nanjing 210019, China)
  • Online:2021-06-25 Published:2021-06-29

摘要: 建立基质匹配曲线校正-高效液相色谱-串联质谱法同时测定干性食品包装纸中安赛蜜、糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、新橙皮苷二氢查尔酮、纽甜、甜菊糖苷8 种甜味剂含量。以原纸为对象配制基质匹配溶剂,采用三乙胺缓冲溶液为萃取溶剂对样品进行超声提取,经XDB-C18色谱柱分离,甲醇-5 mmol/L乙酸铵溶液为流动相进行梯度洗脱,以电喷雾离子源正负离子切换多反应监测模式进行质谱检测,采用外标法定量。结果表明:在优化条件下,8 种甜味剂在各自质量浓度范围内线性关系良好(R2均大于0.995),检出限和定量限分别在0.13~2.50 mg/kg和0.43~8.33 mg/kg之间,加标回收率在85.49%~108.81%之间,相对标准偏差在2.24%~6.03%之间;对市售不同品牌共10 个不同规格糖果、巧克力用干性食品包装纸进行检测分析,结果显示有1 个样品中检测出含有安赛蜜和纽甜,含量分别为0.56 g/kg和0.015 g/kg。本方法具有快捷、高效、准确可靠等特点,可用于干性食品包装纸中8 种甜味剂的同时定量检测。

关键词: 基质匹配溶剂;高效液相色谱-串联质谱;食品包装纸;甜味剂;基质效应

Abstract: A method for simultaneous determination of eight sweeteners in dry food wrappings, acesulfame, saccharin sodium, sodium cyclamate, sucralose, aspartame, neohesperidin dihydrochalcone, neotame, and stevioside, was developed by using matrix matched solvent calibration-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The matrix matching solvent was prepared by using the base paper. The analytes in samples were ultrasonically extracted with triethylamine buffer solution, separated on an XDB-C18 column by gradient elution using methanol-5 mmol/L ammonium acetate solution as the mobile phase. The mass spectrometry was performed in the positive and negative ion switching mode by multiple reaction monitoring mode with an electrospray ion source, and an external standard method was used for quantification. The results showed that under the optimal conditions, the developed method exhibited excellent linearity for the eight sweeteners (R2 ≥ 0.995). The limits of detection (LODs) and quantification (LOQs) were in the range of 0.13–2.50 mg/kg and 0.43–8.33 mg/kg, respectively. The spiked recoveries ranged from 85.49% to 108.81%, with relative standard deviations (RSDs) of 2.24%–6.03%. When the method was used to test ten different commercial dry food wrappings for different brands of candy and chocolate, one sample was detected to contain acesulfame and neotame at 0.56 and 0.015 g/kg, respectively. This method had the advantages of rapidity, high efficiency and good reproducibility, making it a promising candidate for simultaneous quantitative determination of the eight sweeteners in dry food wrappings.

Key words: matrix matched solvent; high performance liquid chromatography-tandem mass spectrometry; dry food wrappings; sweetener; matrix effects

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