UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物

doi:10.7506/spkx1002-6630-20191224-277

食品科学 ›› 2021, Vol. 42 ›› Issue (12): 302-308.doi: 10.7506/spkx1002-6630-20191224-277

UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物

颜伟华，周莹，郭浩炜，王笑笑

（衢州市食品药品检验研究院，浙江衢州 324002）

出版日期:2021-06-25 发布日期:2021-06-29
基金资助:
浙江省衢州市重点实验室项目（衢市科发高[2015]8号）；衢州市专家工作站项目（衢委人才[2018]4号）

Rapid Screening of 27 Plant Growth Regulator and Antibiotic Residues in Bean Sprouts by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

YAN Weihua, ZHOU Ying, GUO Haowei, WANG Xiaoxiao

(Quzhou Institute for Food and Drug Control, Quzhou 324002, China)

Online:2021-06-25 Published:2021-06-29

摘要/Abstract

摘要： 目的：建立超高效液相色谱-串联质谱快速筛查豆芽中27 种植物生长调节剂和抗生素类药物的方法。方法：采用Agilent Eclipse Plus-C18（2.1 mm×100 mm，1.8 μm）色谱柱，以0.01%甲酸为流动相A，甲醇为流动相B，梯度洗脱，流速为0.4 mL/min，质谱选择多反应监测模式对27 种植物生长调节剂和抗生素类药物进行定性和定量检测。结果：该方法线性范围宽、相关性好，R2≥0.998 5；精密度相对标准偏差为1.1%～3.5%（n=6）；方法采用3 个添加水平进行加样回收实验，回收率范围为80.5%～114.9%；检出限为0.5～50 μg/kg，定量限为1.5～150 μg/kg。结论：该方法快速、专属性强、灵敏度高，可同时完成豆芽中27 种植物生长调节剂和抗生素类药物的检测。

关键词: 超高效液相色谱-串联质谱法；豆芽；植物生长调节剂；抗生素类药物

Abstract: Objective: An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for rapid screening of 27 plant growth regulator and antibiotic residues in bean sprouts. Methods: The analytes were separated on an Agilent Eclipse Plus-C18 chromatographic column (2.1 mm × 100 mm, 1.8 μm) using gradient elution with 0.01% formic acid as mobile phase A and methanol as mobile phase B at a flow rate of 0.4 mL/min. Qualitative and quantitative analysis were conducted by tandem mass spectrometry in a multiple reaction monitoring (MRM) mode. Results: The method had a wide linear range and good linearity, with correlation coefficient (R2) ≥ 0.998 5, and the precision expressed as relative standard deviation (RSD) ranged from 1.1% to 3.5% (n = 6). The recoveries at three spiked concentrations ranged from 80.5% to 114.9%. The limits of detection (LOQ) and limits of quantitation (LOQ) were 0.5–50 and 1.5–150 μg/kg, respectively. Conclusion: This method is rapid, accurate and highly sensitive, and can be used for simultaneous detection of the 27 plant growth regulator and antibiotic residues in bean sprouts.

Key words: ultra-high performance liquid chromatography-tandem mass spectrometry; bean sprouts; plant growth regulators; antibiotics

中图分类号:

TS207.3

颜伟华，周莹，郭浩炜，王笑笑. UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物[J]. 食品科学, 2021, 42(12): 302-308.

YAN Weihua, ZHOU Ying, GUO Haowei, WANG Xiaoxiao. Rapid Screening of 27 Plant Growth Regulator and Antibiotic Residues in Bean Sprouts by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2021, 42(12): 302-308.

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UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物

Rapid Screening of 27 Plant Growth Regulator and Antibiotic Residues in Bean Sprouts by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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