食品科学 ›› 2021, Vol. 42 ›› Issue (5): 177-186.doi: 10.7506/spkx1002-6630-20200311-180

• 营养卫生 • 上一篇    下一篇

紫苏籽肽对环磷酰胺致性功能损伤大鼠的改善作用

李明亮,姜盛,郭颖,陈亮,王雨辰,蔡木易,谷瑞增,魏颖   

  1. (1.中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心,北京 100015;2.华狐生物科技(中国)有限公司,辽宁 沈阳 110000)
  • 出版日期:2021-03-15 发布日期:2021-03-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400604)

Ameliorative Effect of Perilla Seed Peptide on Cyclophosphamide-Induced Sexual Function Impairment of Rats

LI Mingliang, JIANG Sheng, GUO Ying, CHEN Liang, WANG Yuchen, CAI Muyi, GU Ruizeng, WEI Ying   

  1. (1. Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food & Fermentation Industries, Beijing 100015, China; 2. Huahu Biotechnology (China) Co. Ltd., Shenyang 110000, China)
  • Online:2021-03-15 Published:2021-03-29

摘要: 目的:研究紫苏籽肽对环磷酰胺造模大鼠性功能损伤的改善作用。方法:80 只SPF级6 周龄雄性SD大鼠随机分为8 组,每组10 只。除空白组外,其余各组大鼠通过注射20 mg/(kg mb·d)环磷酰胺造模,随后各干预组大鼠经不同剂量的紫苏籽肽和紫苏籽蛋白灌胃处理。用酶联免疫吸附测定、实时荧光定量聚合酶链式反应、苏木精-伊红染色分别检测大鼠血清中相关细胞因子、蛋白和激素水平,睾丸中相关基因表达量以及睾丸和肾脏组织的病理形态变化,同时检测睾丸和肾脏中超氧化物歧化酶活力和丙二醛含量,测定睾丸和肾脏以及免疫器官的脏器指数。结果:模型组与空白组相比,大鼠血清睾酮、游离睾酮、促黄体生成素、卵泡刺激素、白细胞介素2、干扰素γ、免疫球蛋白G水平均显著下降,相关基因表达量显著降低,超氧化物歧化酶活力显著降低,丙二醛含量显著升高,脏器指数显著下降(P<0.05);不同剂量紫苏籽肽和蛋白处理组大鼠的上述指标均有不同程度改善,其中紫苏籽肽对于睾丸和肾脏氧化应激状态以及血清激素水平的影响显著(P<0.05)。结论:紫苏籽肽可以有效改善环磷酰胺造成的大鼠生殖损伤状况从而达到改善性功能的作用,并提高大鼠血清白细胞介素2、干扰素γ、免疫球蛋白G水平和免疫器官脏器指数。

关键词: 紫苏籽肽;睾丸;睾酮;性功能;抗氧化

Abstract: Purpose: To study the ameliorative effect of Perilla seed peptide on cyclophosphamide-induced sexual function impairment in rats. Methods: Eighty specific pathogen-free (SPF) 6-week-old male Sprague Dawley (SD) rats were randomly divided into eight groups of ten rats each. All animals except those in the control group were injected with 20 mg/(kg mb · d) of cyclophosphamide, and then treated with perilla seed protein or peptide at various concentrations by gavage. Enzyme linked immunosorbent assay, real-time quantitative polymerase chain reaction (qPCR) and hematoxylin-eosin (HE) staining were used to evaluate the levels of cytokines, proteins and hormones in the serum of rats, gene expression in the testis, and pathological changes of testis and kidney tissues. Meanwhile, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in testis and kidney were determined, and testis, kidney and immune organ indexes were also measured. Results: Compared with the control group, the levels of serum testosterone, free testosterone, luteinizing hormone, follicle stimulating hormone, interleukin 2 (IL-2), interferon γ (IFN-γ) and immunoglobulin G (IgG) in rats in the model group were significantly decreased, the expression of relevant genes and SOD activity were significantly reduced, and MDA content was significantly increased (P < 0.05). Both Perilla seed peptide and protein could improve the above parameters at various degrees. Perilla seed peptide had more significant effects on testicular and renal oxidative stress and serum hormone levels (P < 0.05). Conclusion: Perilla seed peptide can effectively ameliorate cyclophosphamide-induced reproductive damage and consequently improve sexual function in rats and increase the serum levels of IL-2, IFN-γ and IgG as well as immune organ indices.

Key words: perilla seed peptide; testis; testosterone; sexual function; antioxidant

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