食品科学 ›› 2021, Vol. 42 ›› Issue (14): 165-169.doi: 10.7506/spkx1002-6630-20200628-358

• 成分分析 • 上一篇    下一篇

电感耦合等离子质谱法测定母乳中10 种矿物元素

王亚玲,赵军英,乔为仓,张明辉,刘妍,陈历俊   

  1. (北京三元食品股份有限公司,国家母婴乳品健康工程技术研究中心,北京市乳品工程技术研究中心,母乳研究技术创新中心,北京 100163)
  • 发布日期:2021-07-27
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2019YFF0216702;2018YFC1604301-03); 大兴区重大科技成果转化项目(2020006);北京市科技专项(Z191100001719006); 奶牛产业技术体系北京市创新团队项目(BAIC06-2020)

Determination of 10 Elements in Breast Milk by Inductively Coupled Plasma-Mass Spectrometry

WANG Yaling, ZHAO Junying, QIAO Weicang, ZHANG Minghui, LIU Yan, CHEN Lijun   

  1. (Beijing Sanyuan Foods Co. Ltd., National Engineering Center of Dairy for Maternal and Child Health, Beijing Engineering Research Center of Dairy, Technical Innovation Center of Human Milk Research, Beijing 100163, China)
  • Published:2021-07-27

摘要: 建立电感耦合等离子体质谱(inductively coupled plasma-mass spectrometry,ICP-MS)的同时测定母乳中K、Ca、Na、Mg、P、Cu、Mn、Zn、Fe、Se共10 种矿物元素的分析方法。以浓硝酸和双氧水为混合试剂,采用密闭高压消解技术对母乳样品消解后,用ICP-MS检测,利用动态反应池技术有效地消除了分析过程中质谱的干扰,选取Rh作为内标元素校正基体效应和信号漂移。标准曲线用外标法定量,以相对标准偏差考察方法的重复性,以标准物质考察方法的准确性。实验测定的10 种元素线性回归方程的相关系数均大于0.999 0,回收率范围为80.00%~118.19%,对照分析的奶粉成分分析标准物质(GBW10017)和脱脂奶粉成分分析标准物质(GBW08509)的K、Ca、Na、Mg、P、Cu、Mn、Zn、Fe、Se测定值均在标示值范围内,相对标准偏差均小于10.0%。结果表明,实验方法具有较好的准确度和精密度,适用于测定批量母乳的矿物元素。

关键词: 母乳;矿物元素;电感耦合等离子体质谱;密闭高压消解

Abstract: A method for simultaneous and quantitative determination of 10 elements including K, Ca, Na, Mg, P, Cu, Mn, Zn, Fe and Se in breast milk was established by inductively coupled-plasma mass spectrometry (ICP-MS). Samples were digested by using a mixture of HNO3 and H2O2 in a closed high pressure vessel before detection by ICP-MS. The mass spectral interferences caused by polyatomic ions were effectively eliminated by using a dynamic reaction cell. The element Rh was used as an internal standard element to correct the matrix effect and signal drift. The external standard calibration method was used for quantification, and relative standard deviation (RSD) was used to examine the method reproducibility. The accuracy of this method was evaluated by using standard reference materials. The correlation coefficients of the linear regression equations for the 10 elements were all above 0.999 0, and the recoveries ranged from 80.00% to 118.19%. The measured values for standard reference materials of milk powder (GBW10017) and defatted milk powder (GBW08509) were in agreement with the certified values, with RSDs below 10.0%. These results showed that this method was precise, accurate and suitable for the determination of mineral elements in massive breast milk samples.

Key words: breast milk; mineral elements; inductively coupled-plasma mass spectrometry; sealed high pressure digestion

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