鳗鱼骨胶原肽钙螯合物的制备及其稳定性和Caco-2吸收特性

doi:10.7506/spkx1002-6630-20200706-078

食品科学 ›› 2020, Vol. 41 ›› Issue (24): 1-8.doi: 10.7506/spkx1002-6630-20200706-078

• 食品化学 • 下一篇

鳗鱼骨胶原肽钙螯合物的制备及其稳定性和Caco-2吸收特性

钱跃威，徐瀚麟，吕奇晏，陈智超，滕慧，陈雷

（福建农林大学食品科学学院，福建福州 350002）

出版日期:2020-12-25 发布日期:2020-12-28
基金资助:
国家自然科学基金青年科学基金项目（31801459）

Preparation and Stability of Calcium-Chelating Peptide and Its Absorption Characteristics in Caco-2 Cells

QIAN Yuewei, XU Hanlin, LÜ Qiyan, CHEN Zhichao, TENG Hui, CHEN Lei

(College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China)

Online:2020-12-25 Published:2020-12-28

摘要/Abstract

摘要： 结合胃蛋白酶、胰蛋白酶和胰凝乳蛋白酶制备具有高钙结合能力的鳗鱼胶原蛋白肽（eel bone collagen peptide，EBCP）。通过优化确定制备EBCP-Ca的最佳条件为40 ℃、pH 7.5、40 min、多肽与钙质量比5∶1，此条件下钙螯合率达80%以上。荧光光谱、傅里叶变换红外光谱和扫描电子显微镜检测结果表明，胶原蛋白肽的羧基和氨基氮原子可能会螯合钙，从而形成EBCP-Ca。EBCP-Ca可在不同温度、pH值条件下保持稳定性并进行模拟胃肠道消化，同时EBCP-Ca对Caco-2细胞呈现低毒性作用。另外，使用Caco-2细胞单层模型验证EBCP-Ca在体外的钙摄取率，研究EBCP-Ca浓度和培养时间对Caco-2细胞钙离子质量浓度和碱性磷酸酶（alkaline phosphatase，AKP）活性的影响。结果表明，EBCP-Ca能提高AKP活性，进而有利于促进人体胃肠道中钙的吸收。该研究为开发新的钙补充剂和鳗鱼骨的高价值利用提供了一定科学依据。

关键词: 鳗鱼骨；骨钙结合肽；稳定性；细胞吸收

Abstract: In this study, pepsin, trypsin, and chymotrypsin were used in combination to prepare eel bone collagen peptide (EBCP) with high calcium-binding ability. The optimal preparation conditions of EBCP-calcium chelate that provided maximum calcium chelation rate of over 80% were determined as follows: 40 ℃, pH 7.5, 40 min, and mass ratio of peptide to calcium 5:1. Fluorescence, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopic (SEM) observation indicated that the carboxyl and amino nitrogen atoms of EBCP could chelate calcium to form EBCP-Ca. EBCP-Ca was stable toward temperature, pH and simulated gastrointestinal digestion. In addition, this chelate exhibited low toxicity to mouse Caco-2 cells. In addition, the Caco-2 cell monolayer model was used to evaluate the calcium uptake-promoting activity of EBCP-Ca in vitro. The effects of EBCP-Ca concentration and culture time on the calcium content and alkaline phosphatase (AKP) activity in Caco-2 cells were investigated. It was demonstrated that EBCP-Ca enhanced the AKP activity, thereby promoting the absorption of calcium in the human gastrointestinal tract. This research provides a scientific basis for the development of new calcium supplements and the high-value utilization of eel bones.

Key words: eel bone; calcium-binding peptide; stability; cell absorption

中图分类号:

TS254.9

钱跃威，徐瀚麟，吕奇晏，陈智超，滕慧，陈雷. 鳗鱼骨胶原肽钙螯合物的制备及其稳定性和Caco-2吸收特性[J]. 食品科学, 2020, 41(24): 1-8.

QIAN Yuewei, XU Hanlin, LÜ Qiyan, CHEN Zhichao, TENG Hui, CHEN Lei. Preparation and Stability of Calcium-Chelating Peptide and Its Absorption Characteristics in Caco-2 Cells[J]. FOOD SCIENCE, 2020, 41(24): 1-8.

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鳗鱼骨胶原肽钙螯合物的制备及其稳定性和Caco-2吸收特性

Preparation and Stability of Calcium-Chelating Peptide and Its Absorption Characteristics in Caco-2 Cells

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