食品科学 ›› 2021, Vol. 42 ›› Issue (24): 175-182.doi: 10.7506/spkx1002-6630-20200819-255

• 成分分析 • 上一篇    

不同质量分数食盐腌渍艳红辣椒的风味物质分析

吴凯,覃业优,蒋立文,周辉,邓放明,李跑,王蓉蓉   

  1. (1.湖南农业大学食品科学技术学院,湖南 长沙 410128;2.食品科学与生物技术湖南省重点实验室,湖南 长沙 410128;3.湖南坛坛香食品科技有限公司,湖南 长沙 410300)
  • 发布日期:2021-12-30
  • 基金资助:
    国家特色蔬菜产业技术体系发酵加工岗位科学家项目(CARS-24-E-02)

Analysis of Flavor Substances in Pickled Peppers (Capsicum annuum L. cv. Yanhong) with Different Salt Contents

WU Kai, QIN Yeyou, JIANG Liwen, ZHOU Hui, DENG Fangming, LI Pao, WANG Rongrong   

  1. (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha 410128, China;3. Hunan Tantanxiang Food Technology Co. Ltd., Changsha 410300, China)
  • Published:2021-12-30

摘要: 为考察不同质量分数食盐对腌渍艳红辣椒品质的影响,通过设计6%、9%、12%、15%、18%?5?种不同质量分数食盐腌渍1?a的艳红辣椒,比较腌渍辣椒的感官评定、氨基酸态氮、有机酸、总酸含量及气相离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)测定挥发性成分的差异性。结果表明:5?种不同质量分数食盐腌渍辣椒的感官评分分别为5.75、7.66、8.51、7.99、7.78?分。5?种不同质量分数食盐腌渍辣椒总酸含量分别为(5.431±0.014)、(4.575±0.009)、(4.227±0.012)、(4.125±0.013)、(3.882±0.005)g/kg,不同质量分数食盐腌渍的辣椒之间总酸有显著差异(P<0.05)。氨基酸态氮质量分数分别为(0.165?5±0.000?5)%、(0.128?5±0.001?2)%、(0.086?2±0.000?8)%、(0.056?3±0.001?0)%、(0.033?6±0.000?5)%,不同质量分数食盐腌渍的辣椒之间氨基酸态氮含量有显著差异(P<0.05)。利用高效液相色谱检测腌渍辣椒的6?种有机酸,分别是草酸、酒石酸、乳酸、苹果酸、乙酸、柠檬酸。随着食盐质量分数的增加,有机酸的含量不断减少,其中草酸含量最高,在食盐质量分数12%以上处理的样品中未检测出酒石酸。采用GC-IMS分析其挥发性物质气味指纹图谱差异,结果表明食盐质量分数为6%时乳酸乙酯、DL-白氨酸乙酯、乙酸异戊酯、乙酸乙酯、4-羟基丁酸内酯、甲乙酮、异戊醛、2-乙酰基噻唑等风味物质非常多,随着食盐质量分数增加,上述物质减少,而释放出的乙酸乙酯、2-甲基丙酸乙酯、3-甲基-3-丁烯-1-醇、2-甲基丁酸乙酯、2-辛醇、3-甲基-1-戊醇、正戊醇、2,3-丁二酮、3-戊酮、2-戊基呋喃等风味物质会逐渐增多,风味差异明显。相比其他食盐质量分数,9%~12%质量分数食盐腌渍的艳红辣椒风味品质较好,可直接用于剁辣椒的加工调味,对辣椒加工具有一定的指导意义。

关键词: 食盐质量分数;腌渍;艳红辣椒;挥发性成分;有机酸;气相离子迁移谱

Abstract: In order to investigate the influence of different salt contents on the quality of pickled peppers, this study compared the sensory quality, amino nitrogen, organic acid and total acid contents and volatile composition as determined by gas chromatography-ion mobility spectroscopy (GC-IMS) of one-year-old pickled peppers with different salt contents (6%, 9%, 12%, 15%, and 18%). The results showed that the sensory scores of the five pickled peppers were 5.75, 7.66, 8.51, 7.99, and 7.78 points, respectively. The contents of total acids in them were (5.431 ± 0.014), (4.575 ± 0.009), (4.227 ± 0.012), (4.125 ± 0.013), and (3.882 ± 0.005) g/kg, respectively, indicating a significant difference from each other (P < 0.05). The contents of amino nitrogen were (0.165 5 ± 0.000 5)%, (0.128 5 ± 0.001 2)%, (0.086 2 ± 0.000 8)%, (0.056 3 ± 0.001 0)%, and (0.033 6 ± 0.000 5)%, respectively, with a significant difference from each other (P < 0.05). A total of six organic acids were detected in all samples by high performance liquid chromatography (HPLC), namely oxalic acid, tartaric acid, lactic acid, malic acid, acetic acid, and citric acid. The contents of organic acids continued to decrease with the increase in salt content. Among them, the content of oxalic acid was the highest. Tartaric acid was not detected in the sample with salt contents equal to or greater than 12%. GC-IMS was used to analyze the difference in the volatile fingerprints of all samples, showing that higher contents of ethyl lactate, ethyl 2-hydroxy-4-methylpentanoate, isoamyl acetate, ethyl acetate, and 4-hydroxyl were found in the sample with 6% salt content. As the salt content increased, the amounts of the above substances decreased, while the amounts of other flavor substances such as ethyl acetate, ethyl 2-methylpropionate, 3-methyl-3-buten-1-ol, ethyl 2-methylbutanoate, 2-octanol, 3-methyl-1-pentanol, n-pentanol, 2,3-butanedione, 3-pentanone and 2-pentylfuran gradually increased, so that an obvious flavor difference occurred. Compared with those with other salt contents, pickled peppers with salt contents of 9%–12% had better flavor quality and could be directly used for the processing and seasoning of chopped hot pepper. The results of this study may be meaningful for guiding the processing of hot pepper.

Key words: salt content; pickled; hot pepper; volatile components; organic acid; gas chromatography-ion mobility spectrometry

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