食品科学 ›› 2021, Vol. 42 ›› Issue (10): 225-234.doi: 10.7506/spkx1002-6630-20200822-296

• 成分分析 • 上一篇    下一篇

基于SPME-GC-MS和电子舌制备羊肉复合调味料

张婷,祁路路,潘道东,杨正苍,党亚丽   

  1. (1.浙江省动物蛋白食品精深加工技术重点实验室,宁波大学食品与药学学院,浙江 宁波 315211;2.宁夏红山河食品股份有限公司,宁夏 吴忠 751100)
  • 出版日期:2021-05-25 发布日期:2021-06-02
  • 基金资助:
    国家自然科学基金面上项目(31771945);宁夏重点研发计划项目(2019BDE02002); 浙江省重点研发计划项目(2020C02035;2018C02051);宁波市自然科学基金项目(2018A610338)

Preparation of Compound Seasoning from Lamb Meat: Analysis of Characteristic Flavor Compounds by SPME-GC-MS and Electronic Tongue

ZHANG Ting, QI Lulu, PAN Daodong, YANG Zhengcang, DANG Yali   

  1. (1. Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China; 2. Ningxia Hongshanhe Food Co. Ltd., Wuzhong 751100, China)
  • Online:2021-05-25 Published:2021-06-02

摘要: 为制备羊肉风味复合调味料,通过固相微萃取-气相色谱-质谱(solid-phase microextraction-gas chromatography-mass spectrometry,SPME-GC-MS)联用技术对内蒙古羔羊前腿肉和后腿肉的挥发性风味物质进行研究。采用分步酶解法处理羊后腿肉,联合使用β-环糊精和姜进行脱膻处理,并对酶解液进行美拉德反应,结合SPME-GC-MS、电子舌和感官评价分析羊肉酶解液和美拉德反应液的特征风味物质,并以美拉德反应液为风味基料确定最佳羊肉复合调味料。结果表明:羊后腿肉中低风味阈值的醛类、不饱和醇类以及重要酸类化合物的含量较高,且产生腥味的胺类物质和产生刺激性气味的1-丁醇较前腿肉低,表明其更适于制备调味料;酶解液中添加3%姜较5% β-环糊精更利于膻味去除;美拉德反应可增强复合调味料的整体风味,其主要特征风味物质为4-甲基-5-(β-羟乙基)噻唑、2-甲基-3-呋喃硫醇和噻吩-2-硫醇。此研究可为开发高品质肉味调味料提供参考。

关键词: 羊肉;复合调味料;固相微萃取-气相色谱-质谱联用技术;脱膻;电子舌

Abstract: In the preparation of a compound seasoning from lamb meat, we analyzed the volatile flavor compounds in lamb foreleg and hind leg from Inner Mongolia by solid-phase micro-extraction combined with gas chromatography-mass spectrometry (SPME-GC-MS). The hind leg meat was sequentially hydrolyzed with two proteases and simultaneously deodorized with β-cyclodextrin or ginger, and Maillard reaction was carried out on the enzymatic hydrolysate. The characteristic flavor compounds of the hydrolysate and the Maillard reaction products from it were analyzed by SPME-GC-MS, electronic tongue and sensory evaluation. Finally, the formulation of compound seasoning with the Maillard reaction products as the flavor base was optimized. Results showed that the contents of low-flavor threshold aldehydes, unsaturated alcohols and important acids were higher, and the contents of amines responsible for the fishy smell and 1-butanol responsible for the pungent odor in the hind leg were lower than those in the foreleg, indicating the former was more suitable for preparing seasonings than the latter. The odor was more effectively removed by adding 3% ginger rather than 5% β-cyclodextrin. The Maillard reaction could effectively enhance the overall flavor of compound seasonings, and the major characteristic flavor substances in the Maillard reaction products were 4-methyl-5-(β-hydroxyethyl) thiazole, 2-methyl-3-furan mercaptan and thiophene-2-mercaptan. This study can provide a theoretical reference for the development of high-quality meat seasonings.

Key words: lamb; compound seasoning; solid-phase microextraction-gas chromatography-mass spectrometry; deodorization; electronic tongue

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