食品科学 ›› 2021, Vol. 42 ›› Issue (19): 325-333.doi: 10.7506/spkx1002-6630-20200916-198

• 专题论述 • 上一篇    

食品中金刚烷胺残留检测方法新进展

彭娟,赖科洋   

  1. (南昌大学 食品科学与技术国家重点实验室,江西 南昌 330031)
  • 发布日期:2021-11-12
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2019YFC1605502); 南昌大学校级大学生创新创业训练计划项目(7901118190)

Progress in the Development of Detection Methods for Amantadine Residues in Foods

PENG Juan, LAI Keyang   

  1. (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330031, China)
  • Published:2021-11-12

摘要: 金刚烷胺是一种抗病毒药物,滥用后会对消费者健康构成威胁。尽管包括中国和美国在内的许多国家都禁止在家畜和家禽生产中使用金刚烷胺,但目前金刚烷胺仍在被非法使用。本综述简要介绍了金刚烷胺的理化性质、危害、检出事件、检测标准和检测方法。传统检测方法,例如高效液相色谱法、液相色谱-质谱联用法、酶联免疫吸附法、免疫层析法以及化学发光免疫分析法等现在已经被广泛用于检测金刚烷胺,基于抗原抗体反应的新型免疫分析法和基于金刚烷胺结构特点的分子印迹新方法正在受到越来越多的关注,有望在将来得到应用。

关键词: 金刚烷胺;食品;液相色谱-质谱联用法;免疫分析法;分子印迹

Abstract: Amantadine (AMD), an antiviral agent, can cause a threat to consumers’ health when abused. Although AMD has been banned from being used in livestock and poultry in many countries including the USA and China, it is still being used illegally. This article presents a brief introduction to the physicochemical properties and hazards of amantadine, some incidences related to amantadine residues in meat and eggs, the latest standards and the commonly used methods for AMD detection. High performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELSA), immunochromatography, and chemiluminescence immunoassay have been traditionally and widely used for the detection of AMD. Novel immunoassays based on antigen-antibody reaction and molecular imprinting methods based on structural features of amantadine are attracting growing attention and may be used in the future.

Key words: amantadine; food; liquid chromatography-mass spectrometry; immunoassay; molecular imprinting

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