乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用

doi:10.7506/spkx1002-6630-20200927-337

食品科学 ›› 2021, Vol. 42 ›› Issue (17): 27-33.doi: 10.7506/spkx1002-6630-20200927-337

乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用

刘佳宜，李婷婷，励建荣，谢晶，林洪，王轰，申照华，郭晓华

（1.渤海大学食品科学与工程学院，生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心，辽宁锦州 121013；2.大连民族大学生命科学学院，辽宁大连 116600；3.上海海洋大学食品学院，上海 201306；4.中国海洋大学食品科学与工程学院，山东青岛 266100；5.蓬莱京鲁渔业有限公司，山东烟台 265600；6.山东美佳集团有限公司，山东日照 276800）

发布日期:2021-09-29
基金资助:
“十三五”国家重点研发计划重点专项（2018YFD0400601）

Inhibitory Effect of Ethyl Maltol on Quorum Sensing and Spoilage Capacity of Aeromonas salmonicida

LIU Jiayi, LI Tingting, LI Jianrong, XIE Jing, LIN Hong, WANG Hong, SHEN Zhaohua, GUO Xiaohua

(1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; 2. College of Life Science, Dalian Minzu University, Dalian 116600, China; 3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 4. College of Food Science and Technology, Ocean University of China, Qingdao 266100, China; 5. Penglai Jinglu Fishery Co., Ltd., Yantai 265600, China; 6. Shandong Meijia Group Co., Ltd., Rizhao 276800, China)

Published:2021-09-29

摘要/Abstract

摘要： 本研究利用群体感应报告菌株紫色杆菌（Chromobacterium violaceum）026鉴定乙基麦芽酚的抗群体感应活性，通过气相色谱-质谱定量检测分析乙基麦芽酚对杀鲑气单胞菌（Aeromonas salmonida）分泌信号分子的影响。添加外源信号分子，以生物被膜形成量、胞外蛋白酶活力、细菌运动性（群集和泳动迁移直径）为指标，分析乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用。结果表明：乙基麦芽酚对紫色杆菌026以及杀鲑气单胞菌的最小抑菌浓度为0.1 mg/mL，在亚抑菌浓度下，乙基麦芽酚可降低紫色杆菌026产紫色杆菌素的能力；气相色谱-质谱定量检测结果显示，乙基麦芽酚对杀鲑气单胞菌所产群体感应信号分子C12-高丝氨酸内酯的分泌具有明显的抑制作用；且0.08 mg/mL乙基麦芽酚对杀鲑气单胞菌生物被膜形成、胞外蛋白酶活力和细菌运动性（群集和泳动）的抑制率分别为71.2%、69.1%、80.4%和82.1%，且抑制作用具有浓度依赖性。由此可见，乙基麦芽酚对杀鲑气单胞菌具有较强的群体感应抑制效果，有望作为群体感应抑制剂用于水产品保鲜。

关键词: 乙基麦芽酚；杀鲑气单胞菌；群体感应；抑制剂

Abstract: In this research, the anti-quorum sensing activity of ethyl maltol was evaluated using the quorum sensing reporter strain Chromobacterium violaceum 026 (CV026). The effect of ethyl maltol on the secretion of signal molecules in Aeromonas salmonioida was detected by gas chromatography-mass spectrometry (GC-MS). The inhibitory effect of ethyl maltol on the quorum sensing and spoilage capacity of Aeromonas salmonida in the presence of exogenous signaling molecules was determined by measuring the biofilm formation ability, extracellular protease activity and swarming and swimming motilities. The results showed that the minimum inhibitory concentration of ethyl maltol against CV026 and Aeromonas salmonioida was 0.1 mg/mL, and ethyl maltol could reduce the violacein-producing ability of CV026 at the sub-minimum inhibitory concentration (sub-MIC). According to GC-MS analysis, ethyl maltol significantly reduced the production of the quorum sensing signal molecule C12-HSL by Aeromonas salmonida. The biofilm formation, extracellular protease activity and swarming and swimming motilities of Aeromonas salmonida were inhibited by 71.2%, 69.1%, 80.4% and 82.1% by 0.08 mg/mL ethyl mathol, respectively, and the inhibitory effect was dose-dependent. Therefore, ethyl maltol has an obvious inhibitory effect on the quorum sensing of Aeromonas salmonida and can be developed as a quorum sensing inhibitor for the preservation of aquatic products.

Key words: ethyl maltol; Aeromonas salmonicida; quorum sensing; inhibitor

中图分类号:

TS201.3

刘佳宜，李婷婷，励建荣，谢晶，林洪，王轰，申照华，郭晓华. 乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用[J]. 食品科学, 2021, 42(17): 27-33.

LIU Jiayi, LI Tingting, LI Jianrong, XIE Jing, LIN Hong, WANG Hong, SHEN Zhaohua, GUO Xiaohua. Inhibitory Effect of Ethyl Maltol on Quorum Sensing and Spoilage Capacity of Aeromonas salmonicida[J]. FOOD SCIENCE, 2021, 42(17): 27-33.

[1]	相启森董闪闪范刘敏马云芳白艳红. 紫外发光二极管对食品接触材料的杀菌动力学及影响因素[J]. 食品科学, 0, (): 0-0.
[2]	韩国强孙协平吴鹏飞陈今朝陈春王庆. 基于高通量测序分析复配小曲白酒发酵过程中微生物群落结构及多样性[J]. , 0, (): 0-0.
[3]	肖宇刘洋刘建军卢海强桑亚新孙纪录. 一株高产几丁质脱乙酰酶红球菌的基因组测序及其应用潜力分析[J]. , 0, (): 0-0.
[4]	张高川何超永王崇龙王大慧卫功元. 转录组测序分析氯化钠对普鲁兰生物合成的影响[J]. 食品科学, 0, (): 0-0.
[5]	左乾程黄永光郭敏胡峰尤小龙程平言. 酱香型白酒机械化制曲发酵细菌群落的演替[J]. , 0, (): 0-0.
[6]	赵乐张晓桐刘利军谢水琪靳奇文孟祥晨. 氨基酸对植物乳杆菌KLDS1.0391生长及细菌素合成的影响[J]. , 0, (): 0-0.
[7]	刘璐杨金桃吴江丽唐忠月曾雪峰张鹏程. 发酵鱼酱酸产GABA乳酸菌的分离筛选及发酵特性[J]. , 0, (): 0-0.
[8]	黎瑶依胡小霞黄永光. 茅台镇酱香型白酒酿造环境中真菌菌群多样性分析[J]. 食品科学, 0, (): 0-0.
[9]	杨玲玲蒋艳涂勇刚毕雅雯刘晶杨严俊徐明生. 复合卵白蛋白-大豆分离蛋白对肉糜凝胶特性和微观结构的影响[J]. , 0, (): 0-0.
[10]	山珊黄艳梅赵雪龙陈文瑶刘成伟龙中儿刘道峰. 级联信号转导系统结合免疫层析法检测大肠埃希氏菌O157:H7[J]. , 0, (): 0-0.
[11]	孙海磊董鹏程毛衍伟梁荣蓉杨啸吟张一敏朱立贤罗欣. 单增李斯特菌对食品加工中的胁迫响应及其机制研究进展[J]. , 0, (): 0-0.
[12]	孙天妹刘阳泰王翔董晓璐刘弘李红梅董庆利. 删失数据分析在食源性致病菌污染估计中的应用进展[J]. 食品科学, 0, (): 0-0.
[13]	刘佳宜李婷婷励建荣谢晶林洪王轰申照华郭晓华. 乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用[J]. , 0, (): 0-0.
[14]	莫祯妮熊盈盈邱树毅曾祥勇. 群体感应抑制剂在食品加工中的研究进展[J]. , 0, (): 0-0.
[15]	唐林郭柯宇赖鲸慧李建龙李琴杨勇邹立扣刘书亮. 屠宰分割过程中猪胴体表面及环境的细菌菌相分析[J]. 食品科学, 0, (): 0-0.

乙基麦芽酚对杀鲑气单胞菌群体感应及腐败活性的抑制作用

Inhibitory Effect of Ethyl Maltol on Quorum Sensing and Spoilage Capacity of Aeromonas salmonicida

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价