食品科学 ›› 2021, Vol. 42 ›› Issue (19): 122-128.doi: 10.7506/spkx1002-6630-20201107-073

• 基础研究 • 上一篇    

山药多糖的体外抗氧化活性

陈丽叶,常希光,冯晓光,杨肖飞,刘慧君,范俊峰,陈湘宁   

  1. (1.北京农学院食品科学与工程学院,北京 102206;2.北京裕农优质农产品种植有限公司,北京 101400;3.北京林业大学生物科学与技术学院,北京 100083)
  • 发布日期:2021-11-12
  • 基金资助:
    首都食品质量安全保障项目(Z181100009318001); 现代农业产业技术体系北京市叶类蔬菜产业创新团队项目(BAIC07-2019)

In Vitro Antioxidant Activity of Chinese Yam Polysaccharides

CHEN Liye, CHANG Xiguang, FENG Xiaoguang, YANG Xiaofei, LIU Huijun, FAN Junfeng, CHEN Xiangning   

  1. (1. College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 2. Beijing Unong High-Quality Farm Products Planting Limited Company, Beijing 101400, China; 3. College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China)
  • Published:2021-11-12

摘要: 为探究山药多糖能否抑制低密度脂蛋白(low density lipoprotein,LDL)氧化,本实验提取了山药(Dioscorea opposita)多糖并除杂,分析了多糖的成分及体外抗氧化活性,对多糖能否抑制LDL氧化进行了初步探讨。结果表明,山药多糖的总糖质量分数为(65.79±1.03)%,蛋白质量分数为(5.89±0.43)%,灰分质量分数为(5.51±0.14)%,糖醛酸质量分数为(10.81±1.14)%;4 个主要多糖组分的分子质量分别为933 742、148 878、39 233、17 318 Da;对1,1-二苯基-2-三硝基苯肼自由基、超氧化物自由基和羟自由基清除率的半抑制质量浓度分别为1.06、1.06 mg/mL和0.98 mg/mL;可有效减缓共轭二烯的形成,1.0 μg/mL山药多糖对硫代巴比妥酸反应物的抑制率为CuSO4组的16.28%(P>0.05);但对LDL糖化的抑制作用不大。本研究可为了解山药多糖对LDL氧化的抑制作用提供参考。

关键词: 山药多糖;抗氧化;低密度脂蛋白;糖化

Abstract: The composition and in vitro antioxidant activity of polysaccharides extracted from Chinese yam (Dioscorea opposite Thunb.) were analyzed. Additionally, we tested whether the polysaccharides could inhibit the oxidation of low-density lipoprotein (LDL). The results showed that the purity of the polysaccharides was (65.79 ± 1.03)%, which also contained (5.89 ± 0.43)% protein, (5.51 ± 0.14)% ash and (10.81 ± 1.14)% uronic acid. The molecular masses of the four main polysaccharide components were 933 742, 148 878, 39 233, and 17 318 Da, respectively. The half maximal inhibitory concentration (IC50) values for 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging activity were 1.06, 1.06 and 0.98 mg/mL, respectively. The polysaccharides effectively delayed the formation of conjugated diene during LDL oxidation induced by CuSO4. At a concentration of 1.0 μg/mL, the polysaccharides inhibited 16.28% thiobarbituric acid reactive substance (TBARS) production induced by CuSO4 (P > 0.05), but had little inhibitory effect on LDL glycation. The results of the present study may provide useful information for understanding the inhibitory effects of Chinese yam polysaccharides on LDL oxidation.

Key words: Chinese yam polysaccharides; antioxidant; low-density lipoprotein; glycation

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