超高压预处理对西番莲果皮抗氧化能力及对HepG2细胞氧化损伤保护作用的影响

doi:10.7506/spkx1002-6630-20201113-140

摘要/Abstract

摘要： 酚类化合物以游离态、酯化态和不溶性结合态存在于植物中，本实验通过超高压预处理紫色西番莲果皮（the peel of Passiflora edulis Sims，PEP），研究超高压预处理后PEP游离态、酯化态和结合态酚类化合物的多酚含量、抗氧化活性和细胞保护作用的变化，从而确定超高压预处理对PEP抗氧化能力及对HepG2细胞氧化损伤保护作用的影响。结果表明：经超高压处理后，不仅PEP 3 种形态多酚的提取率显著提升，其游离态多酚和黄酮含量也明显增加，而酯化态和结合态的多酚含量明显减少。另外，超高压预处理后，游离态多酚的2,2-二苯基-1-三硝基苯肼（2,2-diphenyl-1-picrylhydrazyl，DPPH）自由基清除能力、2,2’-联氨-双-(3-乙基苯并噻唑啉-6-磺酸)（2,2’-azino-bis(3-ehtylbenzothiazoline-6-sulfonic acid)，ABTS）阳离子自由基清除能力和Fe3＋还原能力（ferric-reducing antioxidant power，FRAP）显著高于未超高压处理组，且3?种形态多酚的抗氧化能力与其多酚含量呈正相关。细胞内活性氧（reactive oxygen species，ROS）和细胞凋亡实验结果表明各类多酚清除ROS和抑制细胞凋亡的能力与阳性对照组（VC组）差异很小。综上，超高压预处理可以显著提高PEP游离多酚和黄酮含量及其抗氧化能力，可将其用于功能性食品或保健食品工业，提高其应用和经济价值。

关键词: 紫色西番莲；抗氧化；细胞；活性氧；凋亡

Abstract: Phenolics widely exist in plants in free, esterified, and insoluble bound forms. The aim of the present study was to evaluate the changes in phenol contents, antioxidant and in vitro cytoprotective activities against H2O2 induced oxidative damage on HepG2 cells of free, esterified and insoluble bound phenolics in the peel of Passiflora edulis Sims (PEP) after ultra-high pressure (UHP) pretreatment. The results showed that after UHP treatment, not only the extraction rates of free, esterified and insoluble bound phenolics, but also the free phenol and flavonoid contents in the three phenolic extracts significantly increased. However, the phenol contents in the esterified and insoluble bound phenolic extracts significantly decreased. In addition, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2’-azino-bis (3-ehtylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging capacity and ferric reducing antioxidant power (FRAP) of free phenols from the UHP treated PEP were significantly higher than those of the untreated PEP group, and the antioxidant capacity of all phenolic fractions was positively correlated with the phenol contents. The results of intracellular reactive oxygen species (ROS) and apoptosis assay showed that there was little difference in the ROS scavenging capacity or apoptosis between each phenolic fraction and the positive control group (VC). In summary, UHP pretreatment can effectively increase the phenol content, flavonoid content and antioxidant capacity of free phenolic fraction from Passiflora edulis peel, which could be used in functional foods and nutraceuticals thereby improve its application and economic value.

Key words: Passiflora edulis Sims; antioxidant; cell; reactive oxygen species; apoptosis

中图分类号:

TS255.1

孙朋真，曹建新，赵天瑞，程桂广. 超高压预处理对西番莲果皮抗氧化能力及对HepG2细胞氧化损伤保护作用的影响[J]. 食品科学, 2022, 43(1): 83-91.

SUN Pengzhen, CAO Jianxin, ZHAO Tianrui, CHENG Guiguang. Effect of Ultra-high Pressure Pretreatment on Antioxidant Capacity and Cytoprotective Activities against Oxidative Damage on HepG2 Cells of Peel of Passiflora edulis Sims[J]. FOOD SCIENCE, 2022, 43(1): 83-91.

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超高压预处理对西番莲果皮抗氧化能力及对HepG2细胞氧化损伤保护作用的影响

Effect of Ultra-high Pressure Pretreatment on Antioxidant Capacity and Cytoprotective Activities against Oxidative Damage on HepG2 Cells of Peel of Passiflora edulis Sims

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