呼吸代谢参与粉红单端孢侵染厚皮甜瓜果实不同阶段的防御反应

doi:10.7506/spkx1002-6630-20200717-232

食品科学 ›› 2021, Vol. 42 ›› Issue (21): 145-152.doi: 10.7506/spkx1002-6630-20200717-232

呼吸代谢参与粉红单端孢侵染厚皮甜瓜果实不同阶段的防御反应

薛素琳，南米娜，龚迪，王斌，姜红，Dov PRUSKY，毕阳，薛华丽

（1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.甘肃农业大学理学院，甘肃兰州 730070；3.以色列农业研究组织农产品采后科学部，以色列里雄莱锡安 7505101）

出版日期:2021-11-15 发布日期:2021-11-23
基金资助:
国家自然科学基金一般国际（地区）合作研究项目（31861143046）；国家自然科学基金地区科学基金项目（32060566）

Respiratory Metabolism is Involved in Defense Responses to Trichothecium roseum in Muskmelon Fruit at Different Stages of Infection

XUE Sulin, NAN Mina, GONG Di, WANG Bin, JIANG Hong, Dov PRUSKY, BI Yang, XUE Huali

(1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;2. College of Science, Gansu Agricultural University, Lanzhou 730070, China; 3. Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel)

Online:2021-11-15 Published:2021-11-23

摘要/Abstract

摘要： 目的：探讨三羧酸（tricarboxylic acid cycle，TCA）循环和磷酸戊糖途径（pentose phosphate pathway，PPP）在病原真菌侵染果实中的作用。方法：用粉红单端孢（Trichothecium roseum）接种‘玉金香’厚皮甜瓜果实，观察（22±2）℃、相对湿度55%～60%条件下病斑直径的变化，测定接种果实病健交界处组织TCA循环和PPP关键酶活力以及中间产物含量的变化，分析不同侵染阶段TCA循环和PPP发挥的作用。结果：果实病斑直径在接种后24 h内无明显变化，48 h时明显增大，72 h时显著高于对照组（P＜0.05）。与对照组相比，接种粉红单端孢显著降低了早期果实的异柠檬酸脱氢酶活力（P＜0.05），而显著提高了苹果酸脱氢酶（malate dehydrogenase，MDH）活力（P＜0.05），24 h时MDH活力是对照组的2.14 倍；显著提高了早期果实烟酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide，NAD）和还原型烟酰胺腺嘌呤二核苷酸含量（P＜0.05），24 h时分别高出对照组50.37%和50.31%；激活了果实的NAD激酶，显著提高了后期葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶的活力（P＜0.05），48 h时分别是对照组的1.83 倍和2.51 倍；显著促进了后期果实烟酰胺腺嘌呤二核苷酸磷酸和葡萄糖-6-磷酸的合成（P＜0.05）；显著降低了早期和中期果实核糖-5-磷酸异构酶活力和核酮糖-5-磷酸含量（P＜0.05）。结论：粉红单端孢侵染厚皮甜瓜早期诱导了果实组织TCA循环，后期促进了PPP，说明TCA循环参与了更多果实早期的抗病防御，而PPP在后期寄主防御反应中发挥更大的作用。

关键词: 厚皮甜瓜；粉红单端孢；侵染；三羧酸循环；磷酸戊糖途径

Abstract: Objective: To reveal the roles of the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway (PPP) in fruits infected by pathogenic fungi. Methods: Muskmelon fruit (cv. ‘Yujinxiang’) were artificially wounded and inoculated with Trichothecium roseum. The change of lesion diameter was observed during storage at ambient temperature (22 ± 2) ℃ and 55%–60% relative humidity. The changes in the activities of the key enzymes and the contents of intermediate products in the TCA cycle and PPP at the junction of diseased and healthy tissues were measured with the aim of evaluating the roles of the TCA cycle and PPP at different stages of infection. Results: Lesion diameter in infected fruit did not obviously change within 24 hours after inoculation, but then increased greatly at 48 h, and was significantly higher than that of the uninoculated control at 72 h (P < 0.05). At the early stage of infection, the activity of isocitrate dehydrogenase was inhibited significantly (P < 0.05) and the activity of malate dehydrogenase (MDH) was promoted significantly (P < 0.05) compared with the control, showing a 2.14-fold increase at 24 h. Meanwhile, the contents of nicotinamide adenine dinucleotide (NAD) and reduced nicotinamide adenine dinucleotide (NADH) were increased by 50.37% and 50.31% compared with those in the control group at 24 h, respectively. The infection also promoted NAD kinase activity. The activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were enhanced by 1.83 and 2.51 times relative to the control group at 48 h, respectively. The synthesis of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and glucose-6-phosphate was accelerated at the late stage of infection (P < 0.05). In addition, the activity of ribose-5-phosphate isomerase and the content of ribulose-5-phosphate in infected fruit was significantly decreased at the early and middle stages. Conclusion: The TCA cycle in muskmelon fruit is induced at the early stage of Trichothecium roseum infection and PPP is stimulated at the late stage, indicating that the TCA cycle participates in defense responses in infected fruit mainly at the early stage, while PPP plays a more important role in defense responses at the late stage.

Key words: muskmelon; Trichothecium roseum; infection; tricarboxylic acid cycle; pentose phosphate pathway

中图分类号:

TS255.1

薛素琳，南米娜，龚迪，王斌，姜红，Dov PRUSKY，毕阳，薛华丽. 呼吸代谢参与粉红单端孢侵染厚皮甜瓜果实不同阶段的防御反应[J]. 食品科学, 2021, 42(21): 145-152.

XUE Sulin, NAN Mina, GONG Di, WANG Bin, JIANG Hong, Dov PRUSKY, BI Yang, XUE Huali. Respiratory Metabolism is Involved in Defense Responses to Trichothecium roseum in Muskmelon Fruit at Different Stages of Infection[J]. FOOD SCIENCE, 2021, 42(21): 145-152.

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呼吸代谢参与粉红单端孢侵染厚皮甜瓜果实不同阶段的防御反应

Respiratory Metabolism is Involved in Defense Responses to Trichothecium roseum in Muskmelon Fruit at Different Stages of Infection

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