食品科学 ›› 2021, Vol. 42 ›› Issue (19): 202-210.doi: 10.7506/spkx1002-6630-20201124-238

• 营养卫生 • 上一篇    

蜜柚果肉膳食多酚的结构鉴定及抗氧化机理

李汀,邹波,吴继军,徐玉娟,余元善,李璐,傅曼琴,程丽娜   

  1. (广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广东 广州 510630)
  • 发布日期:2021-11-12
  • 基金资助:
    广东省农业技术体系创新团队项目(2020KJ108);广东省农业科学院果蔬加工研究团队项目(201604TD); 潮州市科技计划项目(2019ZX03)

Structural Identification and Antioxidant Mechanism of Dietary Polyphenols in Fruit Pulp of Honey Pomelo

LI Ting, ZOU Bo, WU Jijun, XU Yujuan, YU Yuanshan, LI Lu, FU Manqin, CHENG Lina   

  1. (Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510630, China)
  • Published:2021-11-12

摘要: 蜜柚果肉富含膳食多酚,极具保健价值,但其理论研究不足导致深加工产业受限。本实验以梅州蜜柚为原料,对其果肉膳食多酚进行粗提和表征,并研究其对H2O2诱导的HepG2细胞氧化应激的保护作用及其潜在作用机理。结果显示,在柚果肉膳食多酚粗提物中共鉴定出7 种可精确定量多酚单体:柚皮苷、芸香柚皮苷、野漆树苷、香草酸、甲基橙皮苷、新西兰牡荆苷和阿魏酸,其中柚皮苷含量为50.54 mg/g,显著高于其他6 种多酚含量。新西兰牡荆苷和野漆树苷为首次在蜜柚果肉中发现。质量浓度为20 μg/ mL和100 μg/mL的膳食多酚粗提物对H2O2诱导的HepG2肝癌细胞氧化损伤均具有保护作用,且能降低该细胞中乳酸脱氢酶的释放,降低活性氧的积累,促进核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)从细胞质到细胞核的转运,并上调其靶基因GSTA2、HO-1、GCLC、NQO1的表达。综上,梅州蜜柚果肉膳食多酚可以作为预防氧化应激相关疾病潜在的抗氧化剂。

关键词: 抗氧化;活性氧;膳食多酚;HepG2;核因子E2相关因子2

Abstract: Honey pomelo pulp is rich in dietary polyphenols with great health benefits. However, the deep processing industry of honey pomelo is restricted due to the lack of theoretical research on these phenolic compounds. In this study, we extracted and characterized dietary polyphenols from the fruit pulp of Meizhou honey pomelo, and we further investigated the protective effect and underlying mechanism of the polyphenols on H2O2-induced oxidative stress in HepG2 cells. A total of 7 individual polyphenols were identified in the polyphenol extract: naringin, narirutin, rhoifolin, vanillic acid, methyl hesperidin, vicenin-2, and ferulic acid. The content of naringin was 50.54 mg/g, significantly higher than the contents of the other six phenolic compounds. Vicenin-2 and rhoifolin were identified for the first time in the pulp of pomelo. The dietary polyphenols at concentrations of 20 and 100 μg/mL had a protective effect against H2O2-induced oxidative damage in HepG2 cells, and decreased in lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) accumulation. Our results also revealed that the dietary polyphenols promoted the translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) from the cytoplasm to the nucleus and up-regulated the expression of the genes encoding its target antioxidant enzymes such as quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), glutathione S-transferase A2 (GSTA2), and glutamate-cysteine ligase catalytic subunit (GCLC). Therefore, the dietary polyphenols from honey pomelo pulp could serve as a potential therapeutic agent for oxidative stress-related disorders.

Key words: antioxidant; reactive oxygen species; dietary polyphenols; HepG2; nuclear factor erythroid 2-related factor 2

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