铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II)

doi:10.7506/spkx1002-6630-20201214-170

食品科学 ›› 2021, Vol. 42 ›› Issue (24): 323-328.doi: 10.7506/spkx1002-6630-20201214-170

• 安全检测 • 上一篇

铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II)

程春生，杨德志，李宏，李秋兰，杨亚玲

（1.昆明理工大学食品科学与工程学院，云南昆明 650500；2.昆明理工大学生命科学与技术学院，云南昆明 650500；3.云南省农业科学院农产品加工研究所，云南昆明 650032）

发布日期:2021-12-30
基金资助:
云南省科技人才和平台计划-技术创新人才培养对象项目（202005AD160015）；云南省科技创新及成果转化专项-云南省农科院科技专项（202002AE320007-03）

A Colorimetric Probe for Determination of Trace Pb(II) in Tea Based on Peroxidase-like Activity of Carbon Dots Co-doped with Copper and Iodine

CHENG Chunsheng, YANG Dezhi, LI Hong, LI Qiulan, YANG Yaling

(1. Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China; 2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; 3. Institute of Agro-products Processing, Yunnan Academy of Agricultural Sciences, Kunming 650032, China)

Published:2021-12-30

摘要/Abstract

摘要： 基于Pb(II)对铜、碘掺杂碳点模拟酶的活性调节，建立简单、灵敏测定茶叶中Pb(II)的比色探针新方法。铜、碘掺杂碳点的动力学分析结果表明：其模拟酶催化参数符合典型的米氏方程模型。Pb(II)吸附于铜、碘掺杂碳点表面后，可促进底物3,3’,5,5’-四甲基联苯胺（3,3’,5,5’-tetramethylbenzidine，TMB）的氧化。Pb(II)在0.27～27.02?mg/L范围内呈良好的线性关系。将该方法用于Pb(II)含量的测定，其检出限可达到34.3?μg/L，实际茶叶样品的加标回收率在92.41%～101.85%范围内，满足GB?5009.12—2017《食品中铅的测定》中的检测要求。该方法具有简便、稳定、特异性强等特点，其检出限也低于国家规定的食品中Pb(II)含量限值。

关键词: 铅；铜、碘掺杂碳点；过氧化物模拟酶；比色探针；茶叶

Abstract: Based on the regulation of Pb(II) on the enzyme-like activity of carbon dots co-doped with copper and iodine (Cu, I-CDs), a novel, simple and sensitive colorimetric probe was established for the determination of Pb(II) in tea samples. The kinetic parameters of Cu, I-CD peroxidase-like activity were well fitted to the Michaelis-Menten equation. When it was adsorbed on the surface of Cu, I-CDs, Pb(II) could promote the oxidation of the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The concentration of Pb(II) in the range of 0.27–27.02 mg/L showed a good linear relationship with the formation of oxidized TMB (oxTMB). The detection limit of the developed method was 34.3 μg/L, and the spiked recoveries for actual tea samples were in the range of 92.41%–101.85%. These figures of merit meet the requirements of the national standard GB 5009.12-2017. In conclusion, this method is simple, stable, and specific, and its detection limit is lower than the country’s limit for Pb(II) content in foods.

Key words: lead; carbon dots co-doped with copper and iodine; peroxidase mimic; colorimetric probe; tea

中图分类号:

O652.1

程春生，杨德志，李宏，李秋兰，杨亚玲. 铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II)[J]. 食品科学, 2021, 42(24): 323-328.

CHENG Chunsheng, YANG Dezhi, LI Hong, LI Qiulan, YANG Yaling. A Colorimetric Probe for Determination of Trace Pb(II) in Tea Based on Peroxidase-like Activity of Carbon Dots Co-doped with Copper and Iodine[J]. FOOD SCIENCE, 2021, 42(24): 323-328.

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铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II)

A Colorimetric Probe for Determination of Trace Pb(II) in Tea Based on Peroxidase-like Activity of Carbon Dots Co-doped with Copper and Iodine

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